Team:SDU-Denmark/project-p

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(Difference between revisions)
(Characterization)
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In order to assess the results, a series of standard dilutions of beta-carotene were made. The concentrations were 1mM, 100 µM, 50 µM, 25 µM, 10 µM, 5 µM, 1 µM, 100 nM and 10 nM. The standard dilutions were also measured on the spectrophotometer. The resulting graphs are presented low: <br>
In order to assess the results, a series of standard dilutions of beta-carotene were made. The concentrations were 1mM, 100 µM, 50 µM, 25 µM, 10 µM, 5 µM, 1 µM, 100 nM and 10 nM. The standard dilutions were also measured on the spectrophotometer. The resulting graphs are presented low: <br>
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[[Image:Team-SDU-denmarkStandart betacaroten curves.png |400px|thumb|center|]]
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[[Image:Team-SDU-denmarkStandart betacaroten curves.png |400px|thumb|center|UV-VIS standard curve of different concentrations of beta-carotene]]
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When combining the graphs of the samples and the standard dilutions, it is clear that the spectres are uniform. This similarity of the spectres indicates that the cells actually do produce beta-carotene. The graphs also give a qualitative indication of the amount of beta-carotene produced by the MG1655 and the Top10 ''E. coli'' cells. The resulting graphs are presented below: <br><br>
When combining the graphs of the samples and the standard dilutions, it is clear that the spectres are uniform. This similarity of the spectres indicates that the cells actually do produce beta-carotene. The graphs also give a qualitative indication of the amount of beta-carotene produced by the MG1655 and the Top10 ''E. coli'' cells. The resulting graphs are presented below: <br><br>
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[[Image:Image-Team-SDU-denmarkBetacarotene standarts and stationary samples.png |400px|center]]
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[[Image:Image-Team-SDU-denmarkBetacarotene standarts and stationary samples.png |400px|thumb|center|The graph shows the absorbance of beta-carotene in ''E. coli'' strains MG1655 and TOP10 at different wavelengths ]]
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The graph shows that the beta-carotene production of TOP10 containing PSB1A2 with constitutively active K274210 produces beta-carotene in a concentration just above 5 µM.<br>  
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The graph shows that the beta-carotene production of TOP10 containing pSB1A2 with constitutively active K274210 produces beta-carotene in a concentration just above 5 µM.<br>  
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The MG1655 containing PSB1A2 with constitutively active K274210 produces beta-carotene in a concentration just above 10 µM.<br>  
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The MG1655 containing pSB1A2 with constitutively active K274210 produces beta-carotene in a concentration just above 10 µM.<br>  
All data can be seen under  
All data can be seen under  
[https://static.igem.org/mediawiki/2010/7/7d/Team-SDU-Denmark-UV-Vis_determination_of_Carotenoids_and_polyene_chromophores_.zip Raw data]<br>
[https://static.igem.org/mediawiki/2010/7/7d/Team-SDU-Denmark-UV-Vis_determination_of_Carotenoids_and_polyene_chromophores_.zip Raw data]<br>

Revision as of 16:48, 27 October 2010