Team:SDU-Denmark/project-p

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(Stability assay)
(Growth assay)
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The purpose of this assay is to see if our transformants deviate from the wild type in growth rate. In the growth measurement assay we have measured OD at 550 nm every hour for 12 hours and at hour 24. In the experimental set-up we used no lag phase was observed in any of the measurements.
The purpose of this assay is to see if our transformants deviate from the wild type in growth rate. In the growth measurement assay we have measured OD at 550 nm every hour for 12 hours and at hour 24. In the experimental set-up we used no lag phase was observed in any of the measurements.
The graph below shows the growth of our wild type E. coli strain MG1655, the MG1655/pSB3K3-K343004 and MG1655/pSB1C3-K343004 respectively.<br><br>  
The graph below shows the growth of our wild type E. coli strain MG1655, the MG1655/pSB3K3-K343004 and MG1655/pSB1C3-K343004 respectively.<br><br>  
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[[Image:Team SDU-Denmark OD WT+FlhDCmutCP.JPG|400px|OD of WT+FlhDCmutCP|center]] <br>All data can be seen under [https://static.igem.org/mediawiki/2010/c/c5/Team_SDU-Denmark_Growth_rate_assay_FlhDCmut_CP.zip Raw data]<br><br>
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[[Image:Team SDU-Denmark OD WT+FlhDCmutCP.JPG|400px|thumb|center|Growth curve of ''E.coli'' strain MG1655-pSB1C3-K343004 and MG1655-pSB3K3-K343004 compared with the wild type ''E. coli'' strain MG1655 showing no significant difference between the three curves. No Lag phase observed. All data can be seen under [https://static.igem.org/mediawiki/2010/c/c5/Team_SDU-Denmark_Growth_rate_assay_FlhDCmut_CP.zip Raw data]]]<br><br>
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite contradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343004 suggests that it is highly unfavourable for the bacteria, wherefore it might be expected that the growth of the bacteria congaing this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth are not significantly influenced by the plasmid. The added reproduction load due to the plasmids, might also prolong the lag phase of the bacteria. Whether this is the case can not be concluded based on this experiment as no lag phase was observed in this experiment.
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite contradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343004 suggests that it is highly unfavourable for the bacteria, wherefore it might be expected that the growth of the bacteria congaing this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth are not significantly influenced by the plasmid. The added reproduction load due to the plasmids, might also prolong the lag phase of the bacteria. Whether this is the case can not be concluded based on this experiment as no lag phase was observed in this experiment.
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Revision as of 15:09, 27 October 2010