Team:SDU-Denmark/project-p

From 2010.igem.org

(Difference between revisions)
(UV-Vis determination of beta-carotene and retinal production)
Line 68: Line 68:
===Growth rate assay===  
===Growth rate assay===  
The purpose of this assay is to see if our transformants deviate from the wild type in growth rate. In the growth measurement assay we have measured OD at 550 nm every hour for 12 hours and at hour 24. In the experimental setup we used no lag phase was observed in any of the measurments. <br> The graph below shows the growth of our wild type ''E. coli'' strain MG1655, the MG1655 transformed with K343004 in pSB3K3 and in pSB1C3 respectively. <br><br> <br><br>  
The purpose of this assay is to see if our transformants deviate from the wild type in growth rate. In the growth measurement assay we have measured OD at 550 nm every hour for 12 hours and at hour 24. In the experimental setup we used no lag phase was observed in any of the measurments. <br> The graph below shows the growth of our wild type ''E. coli'' strain MG1655, the MG1655 transformed with K343004 in pSB3K3 and in pSB1C3 respectively. <br><br> <br><br>  
-
[[Image:Team SDU-Denmark OD WT+FlhDCmutCP.JPG|400px|OD of WT+FlhDCmutCP]] <br>[https://static.igem.org/mediawiki/2010/5/56/Team_SDU-Denmark_Growth_rate_assay_no._2_FlhDCmut_CP.zip Raw data]<br><br>
+
[[Image:Team SDU-Denmark OD WT+FlhDCmutCP.JPG|400px|OD of WT+FlhDCmutCP]] <br> [https://static.igem.org/mediawiki/2010/c/c5/Team_SDU-Denmark_Growth_rate_assay_FlhDCmut_CP.zip Raw data]<br><br>
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite conteradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343004 suggests that it is highly unfavorable for the bacteria, wherefore it might be expected that the growth raate of the bacteria containg this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth rates are not significantly influenced by the plasmid.
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite conteradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343004 suggests that it is highly unfavorable for the bacteria, wherefore it might be expected that the growth raate of the bacteria containg this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth rates are not significantly influenced by the plasmid.
Line 134: Line 134:
=== Growth assay ===
=== Growth assay ===
The purpose of this assay is to see if our transformants deviate from the wild type in growth rate. In the growth measurement assay we have measured OD at 550 nm every hour for 12 hours and at hour 24. In the experimental setup we used no lag phase was observed in any of the measurments. <br> The graph below shows the growth of our wild type ''E. coli'' strain MG1655, the MG1655 transformed with K343007 in pSB3T5 and in pSB1C3 respectively. <br><br>  
The purpose of this assay is to see if our transformants deviate from the wild type in growth rate. In the growth measurement assay we have measured OD at 550 nm every hour for 12 hours and at hour 24. In the experimental setup we used no lag phase was observed in any of the measurments. <br> The graph below shows the growth of our wild type ''E. coli'' strain MG1655, the MG1655 transformed with K343007 in pSB3T5 and in pSB1C3 respectively. <br><br>  
-
[[Image:Team SDU-Denmark OD WT+PS.JPG|400px]] <br><br>
+
[[Image:Team SDU-Denmark OD WT+PS.JPG|400px]] <br>[https://static.igem.org/mediawiki/2010/2/21/Team_SDU_Denmark_Growth_rate_assay_2_PS.zip Raw data] <br><br>
-
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite conteradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343007 suggests that it is highly unfavorable for the bacteria, wherefore it might be expected that the growth raate of the bacteria containg this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth rates are not significantly influenced by the plasmid.
+
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite conteradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343007 suggests that it is highly unfavorable for the bacteria, wherefore it might be expected that the growth raate of the bacteria containg this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth rates are not significantly influenced by the plasmid.  
== K343005 ==  
== K343005 ==  
Line 185: Line 185:
===Growth measurement===
===Growth measurement===
The purpose of this assay is to see if our transformants deviate from the wild type in growth rate. In the growth measurement assay we have measured OD at 550 nm every hour for 12 hours and at hour 24. In the experimental setup we used no lag phase was observed in any of the measurments. <br> The graph below shows the growth of our wild type ''E. coli'' strain MG1655, the MG1655 transformed with K343006 in pSB1C3. <br><br>  
The purpose of this assay is to see if our transformants deviate from the wild type in growth rate. In the growth measurement assay we have measured OD at 550 nm every hour for 12 hours and at hour 24. In the experimental setup we used no lag phase was observed in any of the measurments. <br> The graph below shows the growth of our wild type ''E. coli'' strain MG1655, the MG1655 transformed with K343006 in pSB1C3. <br><br>  
-
[[Image: Team SDU-Denmark OD WT+NinaB.JPG|400px|OD of WT + NinaB]] <br><br>
+
[[Image: Team SDU-Denmark OD WT+NinaB.JPG|400px|OD of WT + NinaB]] <br>[https://static.igem.org/mediawiki/2010/6/68/Team_SDU-Denmark_Growth_rate_assay_no._2_NinaB.zip Raw data] <br><br>
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite conteradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343006 suggests that it is highly unfavorable for the bacteria, wherefore it might be expected that the growth raate of the bacteria containg this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth rates are not significantly influenced by the plasmid.
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite conteradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343006 suggests that it is highly unfavorable for the bacteria, wherefore it might be expected that the growth raate of the bacteria containg this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth rates are not significantly influenced by the plasmid.

Revision as of 02:04, 26 October 2010