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Team:Northwestern/Notebook
From 2010.igem.org
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1-6G (e0840: rbs30-gfp-2xterm) | 1-6G (e0840: rbs30-gfp-2xterm) | ||
| - | 3-20M ( | + | 3-20M (K124017: Bacteriophage Lysis Cassette S105, R, and Rz) |
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Revision as of 19:47, 28 June 2010
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6/14-18/10 (Boot Camp)
DNA extracted from kit
Part: [http://partsregistry.org/partsdb/get_part.cgi?part=BBa_I746200]
6/22/10
We poured our Kan, Amp, Tet, Kan+Amp, Kan+Tet, Amp+Tet antibiotic agar plates.
Antibiotic Concentrations
- Amp: 50mg/500ml
- Kan: 31.97mg/500ml
- Tet: 6mg/500ml
6/23/10
Resuspended 12O and 6G DNA from the iGEM Kit and transformed them into our competent cells.
6G = r0010(inducible promoter) 12O = e0840(rbs30-gfp-2xterm)
6/24/10
Ran a gel of our PCR product to make sure that our taq polymerase master mix was working.
Selected single 12O and 6G colonies and transferred them to LB and incubated for 18 hours.
Let 1 12O and 1 6G plate incubate at RT. Let 1 12O and 1 6G plate incubate at 37°C. Intended to observe lawn growth.
6/25/10
Low DNA yield; Plan to redo DNA extraction from Kit
Also, found pink colonies in 12O plates
Incubating 37°C plates over the weekend to acquire lawn.
6/28/10
Kit to Stock
1-12O (r0010: inducible promoter)
1-6G (e0840: rbs30-gfp-2xterm)
3-20M (K124017: Bacteriophage Lysis Cassette S105, R, and Rz)
