Promoters

35S Promoter Team:Nevada/registry submissions

The 35S promoter is frequently used as a constitutive promoter in plant research, primarily Arabidopsis experiments, and has been demonstrated to work in Nicotiana tabacum (Kuluev et al, 2010). This promoter normally drives transcription of the Cauliflower mosaic virus genome and shows no tissue or developmental specificity (Keller et al, 2002). For these reasons, the 2010 Nevada iGEM team modified the 35S promoter to conform to BioBrick standards, providing a reliable constitutive promoter to future iGEM teams wishing to engineer plants. Keller, M., Haas, M., Bureau, M., Geldreich, A. and Yot, P. (2002) Cauliflower mosaic virus: still in the news. Molecular Plant Pathology, 3(6), 419–429. Kuluev, B. R., Knyazev, A. V., Lebedev, P. Ya., Iljassowa, A. A. and Chemeris, A. V. (2010) Construction of Hybrid Promoters of Caulimoviruses and Analysis of Their Activity in Transgenic Plants. Russian Journal of Plant Physiology, Vol. 57, No. 4, 582-589.

We would like to thank the following sponsors for their support in helping us make this project possible. Much thanks to the [http://www.cabnr.unr.edu/Students/Majors.aspx Departments of Biochemistry and Biotechnology] and the [http://www.cabnr.unr.edu/Students/Glance.aspx College of Agriculture, Biotechnology and Natural Resources] for their encouragement and support. Thank you [http://www.unr.edu/inbre/ Nevada INBRE] for over $6,000 in support for supplies and registration costs. Thank you to Associated Students of the Univeristy of Nevada for supporting our fund raising efforts. Thank you to [http://www.promega.com/Catalog/CountrySelect.aspx?returnurl=/Default.asp Promega Co.] for free enzyme donations. Thank you to [http://www.invitrogen.com/site/us/en/home.html?cid=covinvggl89100000002336s& Invitrogen Co.] for a discount on our Vector NTI program.

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