Team:SDU-Denmark/labnotes2

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(Difference between revisions)
(experiment no.2)
(Purification of pSB1A2 containing J13002)
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''Results:''After the purification the concentration of nucleic acid concentration was measured on the Nano Drop. There was made four purification tubes. Only tube 1 and 3 was used for further experiments because the other contained a very low concentration of nucleic acid. The concentration in tube 1 and 3 was 25,72ng/uL and 25,76ng/uL.
''Results:''After the purification the concentration of nucleic acid concentration was measured on the Nano Drop. There was made four purification tubes. Only tube 1 and 3 was used for further experiments because the other contained a very low concentration of nucleic acid. The concentration in tube 1 and 3 was 25,72ng/uL and 25,76ng/uL.
The purified DNA was loaded in a 1,5% agarose gel together with a 10.000bp long ladder (red).<br>
The purified DNA was loaded in a 1,5% agarose gel together with a 10.000bp long ladder (red).<br>
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[[Image:Team-sdu-denmark-Oprenset psb1A2 medJ13002-1.jpg |400px ]]<br>
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[[Image:Team-sdu-denmark-Oprenset psb1A2 medJ13002-1.jpg |300px ]]<br>
PCR was run on the DNA using the VR and VF2 primers wich ensures the only the brick part are amplificated. The pfu polymerase was used but unfortunately i did not get any results since i did not add any MgSO4 to the premix.<br><br>
PCR was run on the DNA using the VR and VF2 primers wich ensures the only the brick part are amplificated. The pfu polymerase was used but unfortunately i did not get any results since i did not add any MgSO4 to the premix.<br><br>
--[[User:Pernm07|Pernm07]] 08:48, 22 July 2010 (UTC)
--[[User:Pernm07|Pernm07]] 08:48, 22 July 2010 (UTC)

Revision as of 19:21, 21 October 2010