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Team:Imperial College London/Templates/ModuleHeader
From 2010.igem.org
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| style="font-size:1.8em;background-color:#ea8828;width:300px;border:20px solid #ea8828;"| Detection Module | | style="font-size:1.8em;background-color:#ea8828;width:300px;border:20px solid #ea8828;"| Detection Module | ||
| style="font-size:1.8em;background-color:#728175;width:300px;border:20px solid #728175;"| Signaling Module | | style="font-size:1.8em;background-color:#728175;width:300px;border:20px solid #728175;"| Signaling Module | ||
Revision as of 11:07, 20 October 2010
| Detection Module | Signaling Module | Fast Response Module |
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| We decided to design a new mechanism for parasite detection - by using the proteases they release. A novel protein bound to the cell surface, with a signalling peptide attached via a protease cleavage site. When the protease comes along, the signal peptide is released, allowing it to activate our signaling module. | To transduce the signal we used a quorum sensing system of a gram positive bacterium. The two component signal transduction system taken from S. pneumoniae transfers our peptide signal into the cell, activating the fast response module. | Our fast response mechanism is based around using an enzymatic amplification step acting upon a presynthesised substrate. This greatly reduces the time required for producing a recognisable output, enabling useful field testing kits. |
| Learn more... | Learn more... | Learn more... |
