Team:Alberta/Kit

From 2010.igem.org

(Difference between revisions)
(GENOMIKON)
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*Require very little expensive equipment (autoclaves, centrifuges, -70C freezers)
*Require very little expensive equipment (autoclaves, centrifuges, -70C freezers)
*Fast
*Fast
-
*Efficicent
+
*Efficient
*Easily shipped
*Easily shipped
*Inexpensive
*Inexpensive
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The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, [[Team:Alberta/BioByte 2 |BioBytes 2.0]]. The BioByte 2.0 standard solved most of the problems.</p><p>BioBytes 2.0 is:
The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, [[Team:Alberta/BioByte 2 |BioBytes 2.0]]. The BioByte 2.0 standard solved most of the problems.</p><p>BioBytes 2.0 is:
*Fast: Parts can be added to a growing construct in 7 minutes.
*Fast: Parts can be added to a growing construct in 7 minutes.
-
*Efficient: It has been modelled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into ''E. coli''.
+
*Efficient: It has been modeled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into ''E. coli''.
*Equipment: It does not require centrifugation or any other expensive equipment inherently.
*Equipment: It does not require centrifugation or any other expensive equipment inherently.
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The other limitations were solved by using innovative protocols and products:
The other limitations were solved by using innovative protocols and products:
*Equipment: Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
*Equipment: Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
-
*Easily Shipped: We will utilize lyophilized competent cells so that cold temperatures are not neccesary.
+
*Easily Shipped: We will utilize lyophilized competent cells so that cold temperatures are not necessary.
*Inexpensive: Based on our [[Team:Alberta/Distribution analysis|distribution analysis]] the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.</p>
*Inexpensive: Based on our [[Team:Alberta/Distribution analysis|distribution analysis]] the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.</p>
<p>
<p>
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'''Reception'''<p>
'''Reception'''<p>
-
When we tested our kit with high school students from the Edmonton area it had a warm reception. They had great things to say about the educational aspect and the scientfic aspect of the kit. They did give us some useful feedback. An in-depth look at this component can be seen on [[Team:Alberta/human_practices#Real_World_Trials|real world trials]].
+
When we tested our kit with high school students from the Edmonton area it had a warm reception. They had great things to say about the educational aspect and the scientific aspect of the kit. They did give us some useful feedback. An in-depth look at this component can be seen on [[Team:Alberta/human_practices#Real_World_Trials|real world trials]].
</p>
</p>
{{Team:Alberta/endMainContent}}
{{Team:Alberta/endMainContent}}

Revision as of 04:13, 26 October 2010

TEAM ALBERTA

GENOMIKON


The Idea

GENOMIKON evolved from a team objective to bring synthetic biology to high school students. This proved to be a tall task as a high school environment has many limitations that don't apply to university research labs.

To work GENOMIKON must be:

  • Require very little expensive equipment (autoclaves, centrifuges, -70C freezers)
  • Fast
  • Efficient
  • Easily shipped
  • Inexpensive

The Innovation

The limitations imposed by the high school requirements forced our team to design a new standard for the assembly and production of DNA parts, BioBytes 2.0. The BioByte 2.0 standard solved most of the problems.

BioBytes 2.0 is:

  • Fast: Parts can be added to a growing construct in 7 minutes.
  • Efficient: It has been modeled to be 96% efficient. We have also used BioBytes 2.0 to create plasmids that have been transformed successfully into E. coli.
  • Equipment: It does not require centrifugation or any other expensive equipment inherently.

The Kit

The other limitations were solved by using innovative protocols and products:

  • Equipment: Expensive micropipetters are replaced with precision droppers, which can accurately dispense small volumes of solutions.
  • Easily Shipped: We will utilize lyophilized competent cells so that cold temperatures are not necessary.
  • Inexpensive: Based on our distribution analysis the kit will be inexpensive to produce and can be sold at costs that are lower than currently used high school biology labs.

[picture of kit buddi]

Safety

GENOMIKON was designed with safety in mind and as such it has many safety features built in.

  • A common lab strain of E. coli DH5α is used. This strain has been used safely in labs around the world and was designed to be safe.
  • Proper safety equipment is not required but will be recommended to further decrease the risks of contamination.
  • The kit will teach safety education to the students.

Reception

When we tested our kit with high school students from the Edmonton area it had a warm reception. They had great things to say about the educational aspect and the scientific aspect of the kit. They did give us some useful feedback. An in-depth look at this component can be seen on real world trials.