"
Team:Freiburg Bioware/NoteBook/Labjournal/September2
From 2010.igem.org
(Difference between revisions)
(→124. labday 19.09.2010) |
(→Continuation with Colony-PCR of pSB1C3_CD and ViralBrick motif Z34C) |
||
| Line 145: | Line 145: | ||
<b>Loading plan:</b> | <b>Loading plan:</b> | ||
<br/> | <br/> | ||
| - | upper lanes | + | <b>upper lanes</b> |
M CD1 CD2 CD3 CD4 CD5 CD6 CD7 CD8 CD9 CD10 +ctrl(CFP) +ctrl(BAP) -ctrl(pAAV_MCS) 11Q1 12Q1 13Q1 13Q2 M | M CD1 CD2 CD3 CD4 CD5 CD6 CD7 CD8 CD9 CD10 +ctrl(CFP) +ctrl(BAP) -ctrl(pAAV_MCS) 11Q1 12Q1 13Q1 13Q2 M | ||
| - | lower lanes | + | <b>lower lanes</b> |
M 11T41 11T42 11T43 11T44 12T41 12T42 12T43 12T44 13T41 13T42 13T43 13T44 14T41 14T42 14T43 14T44 M | M 11T41 11T42 11T43 11T44 12T41 12T42 12T43 12T44 13T41 13T42 13T43 13T44 14T41 14T42 14T43 14T44 M | ||
| + | |||
| + | <br/> | ||
| + | <b>Expected sizes of the PCR products are:</b> | ||
| + | <ul> | ||
| + | <li>Cytosine deaminase (CD) = 1300bp</li> | ||
| + | <li>Z34C loops insertion motif = 220bp</li> | ||
| + | <li>Positive control 1 = CFP = 750bp</li> | ||
| + | <li>Positive control 2 = BAP_587 = 170bp</li> | ||
| + | </ul> | ||
| + | <br /> | ||
| + | <b>Results</b> | ||
| + | |||
| + | |||
Revision as of 11:32, 19 September 2010
September
124. labday 19.09.2010
Continuation with Colony-PCR of pSB1C3_CD and ViralBrick motif Z34C
Investigator: Bea
Comment: Since the colony PCR of the last try did not work exactly the way we expected it, another colony PCR approach with new primers was conducted.
Loading plan:
upper lanes
M CD1 CD2 CD3 CD4 CD5 CD6 CD7 CD8 CD9 CD10 +ctrl(CFP) +ctrl(BAP) -ctrl(pAAV_MCS) 11Q1 12Q1 13Q1 13Q2 M
lower lanes
M 11T41 11T42 11T43 11T44 12T41 12T42 12T43 12T44 13T41 13T42 13T43 13T44 14T41 14T42 14T43 14T44 M
Expected sizes of the PCR products are:
- Cytosine deaminase (CD) = 1300bp
- Z34C loops insertion motif = 220bp
- Positive control 1 = CFP = 750bp
- Positive control 2 = BAP_587 = 170bp
Results
