Team:Cambridge/Notebook/Week3

From 2010.igem.org

(Difference between revisions)
(Monday)
Line 11: Line 11:
Anja, Hannah and Emily had fun writing a draft article for [http://www.sterilin.co.uk/ Sterilin] describing what iGEM is, what we were hoping to accomplish and what Sterilin products we had been using. Peter and Will also created primer sequences for our first experiment. Quite a productive day!
Anja, Hannah and Emily had fun writing a draft article for [http://www.sterilin.co.uk/ Sterilin] describing what iGEM is, what we were hoping to accomplish and what Sterilin products we had been using. Peter and Will also created primer sequences for our first experiment. Quite a productive day!
-
==Tuesday==
+
{{Team:Cambridge/Templates/Day|Day=Tuesday}}
-
* Duncan provided several E. coli strainsWe plated out TOP10 to create colonies from which to create competent cells for transformation.
+
{{:Team:Cambridge/Templates/rightpic|src=CamNoteWk3_2.jpg}}
-
* We organised all the stock in the lab
+
Duncan gave us several ''E. coli'' strains today, so we started doing real, practical lab work! We plated out TOP10 to create colonies from which to create competent cells for transformation.  
-
* We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns.  He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
+
Duncan also brought us a ''lot'' of boxes of iGEM stocks from previous years. Emily, Peter, Theo and Anja spent the afternoon sorting through that and making towers of boxes in the lab - putting our tower building practice into use.
 +
 
 +
We also met with Dr. Summers to discuss quiescence. He was very encouraging but we have yet to settle IP concerns.  He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
==Wednesday==
==Wednesday==

Revision as of 10:16, 10 August 2010