Team:Newcastle/18 August 2010

From 2010.igem.org

(Difference between revisions)
(Discussion)
 
(One intermediate revision not shown)
Line 40: Line 40:
[[Image:Nanodrop18820104.jpeg|350px]]
[[Image:Nanodrop18820104.jpeg|350px]]
'''Figure 4''': Screenshot of ''yneA'' tube 4
'''Figure 4''': Screenshot of ''yneA'' tube 4
-
 
-
'''Go back to our main [[Team:Newcastle/notebook| Lab book]] page'''
 
==Discussion==
==Discussion==
Line 48: Line 46:
==Conclusion==
==Conclusion==
High DNA concentration was obtained.
High DNA concentration was obtained.
 +
 +
'''Go back to our main [[Team:Newcastle/notebook| Lab book]] page'''
 +
 +
=Double digest of filamentous cell part, pSB1C3 and pGFP-rrnB=
 +
We performed double digests of the filamentous cell part and pSB1C3 using EcoRI and SpeI, ready for ligation.
 +
 +
We also performed double digests of the filamentous cell part and pGFP-rrnB  using EcoRI and NheI, ready for ligation.
 +
 +
==Materials and Protocol==
 +
 +
Please refer to [[Team:Newcastle/Restriction_digests|Restriction digests]].
 +
 +
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 00:05, 28 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map


Contents

Miniprep of filamentous cell part

Aims

The aim of the experiment is to prepare stocks of the plasmid DNA containing our filamentous cell part.

Materials and Protocol

Please refer to protocols mentioned below for materials required:

Results

yneA 1 yneA 2 yneA 3 yneA 4
Concentration of DNA ng/µl 247.3 233.6 456.6 140.0

Table 1: Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.

Nanodrop1882010.jpeg Figure 1: Screenshot of yneA tube 1 Nanodrop18820102.jpeg Figure 2: Screenshot of yneA tube 2 Nanodrop18820103.jpeg Figure 3: Screenshot of yneA tube 3 Nanodrop18820104.jpeg Figure 4: Screenshot of yneA tube 4

Discussion

The concentration of the different tubes range from 140.0 µl/ml to 456.6 µl/ml. The standard value for miniprep extraction is ~150 µg/ml.

Conclusion

High DNA concentration was obtained.

Go back to our main Lab book page

Double digest of filamentous cell part, pSB1C3 and pGFP-rrnB

We performed double digests of the filamentous cell part and pSB1C3 using EcoRI and SpeI, ready for ligation.

We also performed double digests of the filamentous cell part and pGFP-rrnB using EcoRI and NheI, ready for ligation.

Materials and Protocol

Please refer to Restriction digests.


Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon