Team:Michigan

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Latest revision as of 17:50, 27 October 2010

Welcome to the homepage of the University of Michigan Synthetic Biology Team!

Project Abstract

Algae Bioflocculation for Biofuel Production and Bioremediation of Oil Sands Tailings Water

Our team worked on two projects this year. Our first project aims to improve the economics of algal biofuel production by creating a cost efficient microalgae bioflocculant out of E. coli. To achieve this, we over-express Type I pili to increase the cell’s adhesiveness, and also express a chlorovirus protein on the cell surface which specifically binds Chlorella species, a promising algal feedstock for the biofuel industry.

We are also participating in the Oil Sands Initiative and seeking to improve the biodegradation rate of naphthenic acids (NAs), a toxic by-product of the oil extraction process which can linger in the environment for decades. Two Pseudomonas strains have been found to synergistically degrade 95% of NAs. Our project focuses on engineering these Pseudomonas strains to form biofilms in the harsh tailings water environment, which can potentially increase degradation rates by two orders of magnitude, by expressing a self-associating E. coli protein.

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+==Project Abstract==
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+'''Algae Bioflocculation for Biofuel Production and Bioremediation of Oil Sands Tailings Water'''
-The project the team has decided on for this year is in association with the Chemical Engineering department’s joint project to produce hydrocarbons from algae biomass.  This consists of hydrothermal processing of the algae followed by catalytic upgrading of the “bio crude” oil.  Before the hydrothermal processing can take place the algae needs to be concentrated from 1 to 10 g/L to over 250 g/L.  Traditional methods that are used like centrifugation, filtration and chemical flocculation are energy intensive.  We propose creating a bioflocculation circuit in ''Escherichia coli'' to flocculate the algae as an alternative option.  This consists of a “trigger” for flocculation which would then express proteins that cause the surface of the ''E. coli'' cells to become sticky and flocculate with the algae.
+Our team worked on two projects this year. Our first project aims to improve the economics of algal biofuel production by creating a cost efficient microalgae bioflocculant out of E. coli. To achieve this, we over-express Type I pili to increase the cell’s adhesiveness, and also express a chlorovirus protein on the cell surface which specifically binds Chlorella species, a promising algal feedstock for the biofuel industry.
+We are also participating in the Oil Sands Initiative and seeking to improve the biodegradation rate of naphthenic acids (NAs), a toxic by-product of the oil extraction process which can linger in the environment for decades. Two Pseudomonas strains have been found to synergistically degrade 95% of NAs. Our project focuses on engineering these Pseudomonas strains to form biofilms in the harsh tailings water environment, which can potentially increase degradation rates by two orders of magnitude, by expressing a self-associating E. coli protein.
-Our team will also be working on the [[Oil Sands]] Initiative simultaneously. Tailings ponds have become a major ecological concern regarding oil sands operations due to the toxicity of the water. The main cause of toxicity in tailings water has been identified as naphthenic acids (NAs), and thus our strategy will be to these break these organic molecules down via bioremediation to expedite the reclamation process. Two bacteria, ''Pseudomonas fluorescens'' and ''Pseudomonas putida'', were isolated from tailings pond sediments and found to be capable of synergistically degrading >95% of a commercial mixture of NAs resembling those found in the tailings water over a 4 week period. [1] In addition, two other bacteria have shown degradation efficiencies near 100% when in an immobilized cell reactor, which functionally represents a biofilm. Following these findings, the strategy we wish to implement will be to utilize the two naturally adapted ''Pseudomonas'' species and genetically engineer them to optimize their degradation and biofilm formation abilities, then induce them to form a biofilm over a rocky ‘washboard’ which tailings water will be passed over. This washboard should function to clean the water in an economically and environmentally friendly and possibly aesthetically pleasing fashion.
-==References==
-. [http://www.ncbi.nlm.nih.gov/pubmed/17040229 Del Rio, L., Hadwin, A., Pinto, L., MacKinnon, M. and Moore, M. (2006), Degradation of naphthenic acids by sediment micro-organisms. Journal of Applied Microbiology, 101: 1049–1061.]
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