Team:INSA-Lyon/Project/Stage2/Results

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<h3>Results</h3>
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<h2>Results</h2>
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<br><h5><b> Lipidic inclusion in the PHB drop</b></h5>
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<br><h3><font color="purple">Lipidic inclusion in the PHB drop</font></h3><br><br>
<p>We made two double transformations :  <br/>
<p>We made two double transformations :  <br/>
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<li>With pILI1 and 20P: The resultant bacteria synthesize the PHB drops and over synthesize the β-caroten.</li>
<li>With pILI1 and 20P: The resultant bacteria synthesize the PHB drops and over synthesize the β-caroten.</li>
<li>With pILI1 and 20N: The resultant bacteria synthesize the PHB drops and over synthesize the licopen.</li>
<li>With pILI1 and 20N: The resultant bacteria synthesize the PHB drops and over synthesize the licopen.</li>
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<p>When we had obtained the clones, we started four different series of cultures in order to analyzed the will of the lipid when the granule are produced.<br/>
<p>When we had obtained the clones, we started four different series of cultures in order to analyzed the will of the lipid when the granule are produced.<br/>
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<ol id="list_extra">

Revision as of 13:16, 27 October 2010




Results


Lipidic inclusion in the PHB drop



We made two double transformations :

  1. With pILI1 and 20P: The resultant bacteria synthesize the PHB drops and over synthesize the β-caroten.
  2. With pILI1 and 20N: The resultant bacteria synthesize the PHB drops and over synthesize the licopen.


When we had obtained the clones, we started four different series of cultures in order to analyzed the will of the lipid when the granule are produced.

  1. Serie 1 : in LB media with glucose 7% and 2,5µg of RedNile
    1. pILI1 with ampiciline
    2. double transformation with ampiciline
    3. double transformation with ampiciline and kanamycine
    4. The lipid part with kanamycine

  2. Serie 2 : in LB media with 2,5µg of RedNile
    1. pILI1 with ampiciline
    2. double transformation with ampiciline
    3. double transformation with ampiciline and kanamycine
    4. The lipid part with kanamycine

  3. Serie 3 : in LB media with glucose 7%
    1. pILI1 with ampiciline
    2. double transformation with ampiciline and kanamycine
    3. The lipid part with kanamycine

  4. Serie 4 : in LB media, the double transformation with ampiciline and kanamycine


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