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Team:INSA-Lyon/Project/Stage2/Results
From 2010.igem.org
(Difference between revisions)
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<li>Serie 1 : in LB media with glucose 7% and 2,5µg of RedNile<br/> | <li>Serie 1 : in LB media with glucose 7% and 2,5µg of RedNile<br/> | ||
<ol id="list_extra"> | <ol id="list_extra"> | ||
| - | <li> | + | <li> pILI1 with ampiciline</li> |
<li>double transformation with ampiciline</li> | <li>double transformation with ampiciline</li> | ||
<li>double transformation with ampiciline and kanamycine</li> | <li>double transformation with ampiciline and kanamycine</li> | ||
| Line 61: | Line 61: | ||
<li>Serie 2 : in LB media with 2,5µg of RedNile<br/> | <li>Serie 2 : in LB media with 2,5µg of RedNile<br/> | ||
<ol id="list_extra"> | <ol id="list_extra"> | ||
| - | <li> | + | <li> pILI1 with ampiciline</li> |
<li>double transformation with ampiciline</li> | <li>double transformation with ampiciline</li> | ||
<li>double transformation with ampiciline and kanamycine</li> | <li>double transformation with ampiciline and kanamycine</li> | ||
| Line 68: | Line 68: | ||
<li>Serie 3 : in LB media with glucose 7%<br/> | <li>Serie 3 : in LB media with glucose 7%<br/> | ||
<ol id="list_extra"> | <ol id="list_extra"> | ||
| - | <li> | + | <li> pILI1 with ampiciline</li> |
<li>double transformation with ampiciline and kanamycine</li> | <li>double transformation with ampiciline and kanamycine</li> | ||
<li>The lipid part with kanamycine</li> | <li>The lipid part with kanamycine</li> | ||
Revision as of 13:04, 27 October 2010
Results
Lipidic inclusion in the PHB drop
We made two double transformations :
- With pILI1 and 20P: The resultant bacteria synthesize the PHB drops and over synthesize the β-caroten.
- With pILI1 and 20N: The resultant bacteria synthesize the PHB drops and over synthesize the licopen.
When we had obtained the clones, we started four different series of cultures in order to analyzed the will of the lipid when the granule are produced.
- Serie 1 : in LB media with glucose 7% and 2,5µg of RedNile
- pILI1 with ampiciline
- double transformation with ampiciline
- double transformation with ampiciline and kanamycine
- The lipid part with kanamycine
- Serie 2 : in LB media with 2,5µg of RedNile
- pILI1 with ampiciline
- double transformation with ampiciline
- double transformation with ampiciline and kanamycine
- The lipid part with kanamycine
- Serie 3 : in LB media with glucose 7%
- pILI1 with ampiciline
- double transformation with ampiciline and kanamycine
- The lipid part with kanamycine
- Serie 4 : in LB media, the double transformation with ampiciline and kanamycine
