Team:Newcastle/Meetings/4 June 2010

From 2010.igem.org

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{{Team:Newcastle/mainbanner}}
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|Informal Meeting 04.06.2010
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|'''Urease tutorial-recap'''
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|Accessory genes and nickel incorporation necessary more complicated than we thought.
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|Software engineering cycle
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|Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements
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|Specification more detail on the requirements- literature
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|Urease calcium carbonate, optimise for Bacillis
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|Increase expression- what we thought!
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|Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper
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|Accessory genes required!
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|Look over papers from tutorial
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|Sporosarcina pasteurii
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|Biobrick site symbols
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|Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator
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|sequences ...and spacers
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|pSpac
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|ncbi – find the genome – can see the genes
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|Implementation using sequencher
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|Find the sequence of part
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|Assemble parts
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|Biobrick friendly restriction sites
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|Genbank/embl file format
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|Implementation altered by cloning strategy
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|Biobricks RFC- request for comments
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|http://Bbf.openware.org/RFC.html -duplo and lego don’t fit together
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|AP Rachel: video for lego pipette
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|'''1st lab week'''                    Others modelling Filamentous and spidersilk killswitch
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|RC
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|DY
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|AK
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|(HS)
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|'''2nd lab week'''                    Others Modelling glue and lysis quorum sensing
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|JS
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|RB
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|SW
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|PH
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|(YE)
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|Urease both
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==Informal Meeting - 4 June 2010==
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* [[Team:Newcastle/Agendas/4_June_2010|Agenda]]
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'''LAB TRAINING GROUPS:'''
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====Urease tutorial - recap====
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* Accessory genes and nickel incorporation necessary more complicated than we thought.
 +
* Software engineering cycle
 +
* Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements
 +
* Specification more detail on the requirements- literature
 +
* Urease calcium carbonate, optimise for Bacillis
 +
* Increase expression- what we thought!
 +
* Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper
 +
* Accessory genes required!
 +
* Look over papers from tutorial
 +
* Sporosarcina pasteurii
 +
* Biobrick site symbols
 +
* Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers
 +
* pSpac
 +
* ncbi – find the genome – can see the genes
 +
* Implementation using sequencher
 +
* Find the sequence of part
 +
* Assemble parts
 +
* Biobrick friendly restriction sites
 +
* Genbank/embl file format
 +
* Implementation altered by cloning strategy
 +
* Biobricks RFC- request for comments
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* [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts duplo and lego don’t fit together]
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* [http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded Video for lego pipette]
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====LAB TRAINING GROUPS:====
* '''Group 1 starts 14th June''': SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)
* '''Group 1 starts 14th June''': SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)
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* '''Group 2 starts 21st June''': JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Glue & lysis)
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* '''Group 2 starts 21st June''': JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Spider silk and filamentous cells)
* All team members working on BioBrick 1 (CaCO3/Urease).
* All team members working on BioBrick 1 (CaCO3/Urease).
* RC working on BioBrick 6 (sequential programming)
* RC working on BioBrick 6 (sequential programming)
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* Team members not in the lab training will be working on modeling.
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* Team members not in the lab training will be working on modelling?
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* HS and YE will unfortunately be away for both lab training sessions, hence the brackets
* HS and YE will unfortunately be away for both lab training sessions, hence the brackets
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{{Team:Newcastle/footer}}

Latest revision as of 09:01, 27 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Informal Meeting - 4 June 2010

Urease tutorial - recap

  • Accessory genes and nickel incorporation necessary more complicated than we thought.
  • Software engineering cycle
  • Requirements -> specification-> design-> implementation -> testing -> maintenance ...link back to the requirements
  • Specification more detail on the requirements- literature
  • Urease calcium carbonate, optimise for Bacillis
  • Increase expression- what we thought!
  • Biosynthesis of active BS in the Absence of Known Urease Accessory Proteins: paper
  • Accessory genes required!
  • Look over papers from tutorial
  • Sporosarcina pasteurii
  • Biobrick site symbols
  • Promoter-ribosome- binding- coding sequence –ribosome binding site-coding-ribosome binding coding- terminator sequences ...and spacers
  • pSpac
  • ncbi – find the genome – can see the genes
  • Implementation using sequencher
  • Find the sequence of part
  • Assemble parts
  • Biobrick friendly restriction sites
  • Genbank/embl file format
  • Implementation altered by cloning strategy
  • Biobricks RFC- request for comments
  • [http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_10:_Draft_standard_for_Biobrick_biological_parts duplo and lego don’t fit together]
  • [http://www.youtube.com/watch?v=FRijFX0BpGI&feature=player_embedded Video for lego pipette]

LAB TRAINING GROUPS:

  • Group 1 starts 14th June: SW, D.Y AK, (HS); working on Biobrick 2 & 5 (End of crack & signalling system; Glue & lysis)
  • Group 2 starts 21st June: JS, RB, RC, PH, (YE); working on BioBrick 3 & 4 (Non-target-environment kill switch; Spider silk and filamentous cells)
  • All team members working on BioBrick 1 (CaCO3/Urease).
  • RC working on BioBrick 6 (sequential programming)
  • Team members not in the lab training will be working on modeling.
  • HS and YE will unfortunately be away for both lab training sessions, hence the brackets
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