"

From 2010.igem.org

Revision as of 03:27, 29 June 2010 (view source) Gcxmatt (Talk | contribs) (→Reagents) ← Older edit		Revision as of 03:42, 29 June 2010 (view source) Cyu286 (Talk | contribs) (→Reagents) Newer edit →
Line 64:		Line 64:
-	=='''Reagents'''==	+	==[[Reagents]]==

---

Revision as of 03:42, 29 June 2010

Home	Brainstorm	Team	Acknowledgements	Project	Side Project	Parts	Notebook	Calendar	Protocol	Safety	Links	References	Media	Contact

Contents 1 DNA 1.1 Prepping DNA from the kit plates 1.2 Quikchange (from primers to colonies!) 1.3 Kit to Stock Plasmid 2 Bacterial Work 2.1 Transformation 2.2 O/N Culture 2.3 Preparation of Competent Cells 2.4 LB Media 2.5 Preparing Plates 3 Assembly 3.1 Restriction Enzyme Digests 3.2 3A Assembly 3.3 Ligations 3.4 Mini Prep 4 Microscopy 4.1 Confocal Microscopy 4.2 Fluorescence Microscopy 5 Reagents

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents