Team:Northwestern/Project
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UDP-GlcNAc -> UDP + Chitin | UDP-GlcNAc -> UDP + Chitin | ||
- | === chitinase knockout === | + | === chitinase knockout (may not be necessary) === |
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts | summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts |
Revision as of 23:48, 18 June 2010
Home | Team | Project | Parts | Notebook | Safety | Calendar | Protocol |
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Contents |
Overall project
Signalling (volatile or quorum) -> chitin synthase (fast) && holin (slow)
in chitinase-KO strain e coli
Project Details
volatile cell signalling
phcB -> 3-OH PAME -> phcS -> phcR -(negative control)-> phcA
article: http://jb.asm.org/cgi/reprint/179/11/3639?view=long&pmid=9171411
non-volatile cell signalling
registry: http://partsregistry.org/Cell-cell_signalling
quorum sensing: LuxR, LuxS, LuxpR
chitin synthase
chitin synthase gene (chs3): http://www.ncbi.nlm.nih.gov/gene/852311
UDP-GlcNAc -> UDP + Chitin
chitinase knockout (may not be necessary)
summary of e coli knockouts: http://openwetware.org/wiki/Escherichia_coli/Knockouts
chitinase gene (chiA): http://www.ncbi.nlm.nih.gov/gene/947837
chiA-knockout strain e coli: http://ecoli.naist.jp/GB6/info.jsp?id=JW3300
holin
registry: http://partsregistry.org/Cell_death