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Week20 10/24/10-10/30/10
From 2010.igem.org
(New page: ===10/24/10=== Timi took out the CP-LacPI plates into the morning and stored them in the cold room. Kevin inoculated overnight cultures and put them in to grow. ---- ===10/25/10=== Kevi...)
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Revision as of 01:55, 27 October 2010
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10/24/10
Timi took out the CP-LacPI plates into the morning and stored them in the cold room.
Kevin inoculated overnight cultures and put them in to grow.
10/25/10
Kevin miniprepped the CP-LacPI cultures and digested them.
10/26/10
Timi treated Tet backbone with phosphatase for more efficient 3A assemblies.
Timi ligated the CP-LacPI digests with GFP into a Tet backbone. They were transformed into ChiA competent cells and plated onto Tet plates.
10/27/10
Kevin inoculated overnight cultures of the CP-LacPI-GFP colonies. They will be miniprepped/digested tomorrow to be screen on an agarose gel. After we successfully obtain the part, we will characterize the CP-LacPI-GFP constructs that we created to find the weakest and strongest constitutive promoter. We will be using a plate reader to characterize the parts.
WIKI FREEZE at 11:59PM ET. Any further work will be documented elsewhere.
