Week11 8/22/10-8/28/10

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Week11 Summary

Finished MAGE cycling and began screening. Assembled Cp-LacPi-RFP combinations for Cp-LacPi characterization. Also continued site directed mutagenesis.


8/22/10

Cycle 13 of MAGE for tqsA Knockouts.


8/23/10

Site-Directed Mutagenesis

Cycles 14 and 15 of MAGE for tqsA Knockouts.


8/24/10

Ligated CP-LacPI-RFP combinations.


8/25/10

Regrew Cycle 15 MAGE cells in liquid culture.


8/26/10

Screened MAGE culture for knockouts.


8/27/10

Screened MAGE culture for knockouts.