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ULB/27 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{ULB_Header_2}} '''Quorum addiction module''' *Extraction of the BBa_R0011 promoter from the iGEM plates. *Electroporation of the first ligations in E. Coli.) |
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*Extraction of the BBa_R0011 promoter from the iGEM plates. | *Extraction of the BBa_R0011 promoter from the iGEM plates. | ||
*Electroporation of the first ligations in E. Coli. | *Electroporation of the first ligations in E. Coli. | ||
| + | |||
| + | '''Module hydrogen''' | ||
| + | * We continue the purification process for the deletion candidate | ||
| + | * We continue to try to obtain other deletion candidates | ||
| + | |||
| + | '''Module homologous recombination''' | ||
| + | * We inserted the cm and kan resistance cassette into the pSB1C3, unfortunately, the kan resistance cassette is cut by some of the enzymes of the assembly standard 10 and can therefore not be used with the pSB1C3 | ||
| + | * We received the primers for gam and bet. We launch the PCR before the weekend. | ||
| + | |||
| + | '''Module heavy metals detection''' | ||
| + | * We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow | ||
| + | * We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow | ||
| + | |||
| + | '''Other''' | ||
| + | * The PCR on pSB1C3 worked but we obtained the pSB1C3 in very low concentrations | ||
Latest revision as of 03:18, 28 October 2010
Quorum addiction module
- Extraction of the BBa_R0011 promoter from the iGEM plates.
- Electroporation of the first ligations in E. Coli.
Module hydrogen
- We continue the purification process for the deletion candidate
- We continue to try to obtain other deletion candidates
Module homologous recombination
- We inserted the cm and kan resistance cassette into the pSB1C3, unfortunately, the kan resistance cassette is cut by some of the enzymes of the assembly standard 10 and can therefore not be used with the pSB1C3
- We received the primers for gam and bet. We launch the PCR before the weekend.
Module heavy metals detection
- We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow
- We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow
Other
- The PCR on pSB1C3 worked but we obtained the pSB1C3 in very low concentrations
