Team:Yale/Our Project/Notebook/Week 8

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iGEM Yale

lab notebook: week 8 (7/26-8/1)

  • Monday 7/26--Efforts to confirm that ligation of phsABC and B0015 actually occurred.
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  • Tuesday 7/27--Diagnostic double digest of likely ligations
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  • Wednesday 7/28--Sequencing confirms ligation success!
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  • Thursday 7/29--First attempt at Q04121 ligation
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  • Friday 7/30--No colonies visible from 7/29 transformation, so returned plates to incubator.
  • Saturday 7/31--Plates from ligation transformation showed growth.
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