Revision as of 23:23, 25 October 2010 by Olchowik (Talk | contribs)

Example Tabs

ExpressIt! - family of expression vectors with defined strength

Key features:

  • 10 different measured RBS variants
  • each variant in both constitutive (J23100 driven) and inducible (pT7 driven) version
  • just one step to clone any protein coding BioBrick and you have expression construct ready
  • wide spectrum of expression strengths
  • ampicillin resistance - this is important for constructs that use T7 promoter. Why? T7 promoter is active ONLY in presence of T7 polymerase. Therefore you have to use a E.Coli strain containing this gene. The most popular choice E.coli BL21, which has a chloramphenicol resistance gene in the genome.
  • high copy ColE1 ori (based on pSB1A2 vector)
  • terminator included in the vector sequence - pSB1A2 based.

Relative strength of ExpressIt! vector family: