http://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&feed=atom&action=historyTeam:Warsaw/Stage1/ExpressIt - Revision history2024-03-28T17:53:49ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&diff=165562&oldid=prevOlchowik at 20:06, 26 October 20102010-10-26T20:06:56Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:06, 26 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 27:</td>
<td colspan="2" class="diff-lineno">Line 27:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Relative strength of ExpressIt! vector family:</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Relative strength of ExpressIt! vector family:</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2010/0/09/ExpressIt.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2010/0/09/ExpressIt.png"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Fig 1. The estimated expression vectors strength relative to pT7.B0034 - the strongest one. Estimation was based on The promoter and RBS measurement. Promoter and RBSes were assumed to be independent of each other (wchich is partly confirmed by our modeling results).</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TemplateBottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TemplateBottom}}</div></td></tr>
</table>Olchowikhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&diff=161608&oldid=prevOlchowik at 15:48, 26 October 20102010-10-26T15:48:46Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:48, 26 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 24:</td>
<td colspan="2" class="diff-lineno">Line 24:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>terminator included in the vector sequence - <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> based.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>terminator included in the vector sequence - <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> based.</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Relative strength of ExpressIt! vector family:</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Relative strength of ExpressIt! vector family:</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2010/0/09/ExpressIt.png"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2010/0/09/ExpressIt.png"></div></td></tr>
</table>Olchowikhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&diff=161602&oldid=prevOlchowik at 15:48, 26 October 20102010-10-26T15:48:26Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:48, 26 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 10:</td>
<td colspan="2" class="diff-lineno">Line 10:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ExpressIt! - family of expression vectors with defined strength</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ExpressIt! - family of expression vectors with defined strength</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For your convenience we assembled a set of expression vectors. This year we prepared two tasty flavours: J23100 base and pT7 based vectors. Those promoters are available with 10 different RBS sequences (all that we could find in the registry). For constitutive protein expression use J32100 based vectors. T7promoter is stronger than J23100, but it is active only in strains harbouring T7 polymerase. Usually expression of T7polymerase is inducible therefore pT7 is a good choice for strong inducible promoter. For approximate guidelines about expression strength of our vectors see Figure 1. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For your convenience we assembled a set of expression vectors. This year we prepared two tasty flavours: J23100 base and pT7 based vectors. Those promoters are available with 10 different RBS sequences (all that we could find in the registry). For constitutive protein expression use J32100 based vectors. T7promoter is stronger than J23100, but it is active only in strains harbouring T7 polymerase. Usually expression of T7polymerase is inducible therefore pT7 is a good choice for strong inducible promoter. For approximate guidelines about expression strength of our vectors see Figure 1. <ins class="diffchange diffchange-inline"><br></ins><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Olchowikhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&diff=161597&oldid=prevOlchowik at 15:48, 26 October 20102010-10-26T15:48:08Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:48, 26 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 10:</td>
<td colspan="2" class="diff-lineno">Line 10:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ExpressIt! - family of expression vectors with defined strength</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ExpressIt! - family of expression vectors with defined strength</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For your convenience we assembled a set of expression vectors. This year we prepared two tasty flavours: J23100 base and pT7 based vectors. Those promoters are available with 10 different RBS sequences (all that we could find in the registry). For constitutive protein expression use J32100 based vectors. T7promoter is stronger than J23100, but it is active only in strains harbouring T7 polymerase. Usually expression of T7polymerase is inducible therefore pT7 is a good choice for strong inducible promoter. For <del class="diffchange diffchange-inline">approimate </del>guidelines about expression <del class="diffchange diffchange-inline">strenght </del>of our vectors see Figure 1. <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For your convenience we assembled a set of expression vectors. This year we prepared two tasty flavours: J23100 base and pT7 based vectors. Those promoters are available with 10 different RBS sequences (all that we could find in the registry). For constitutive protein expression use J32100 based vectors. T7promoter is stronger than J23100, but it is active only in strains harbouring T7 polymerase. Usually expression of T7polymerase is inducible therefore pT7 is a good choice for strong inducible promoter. For <ins class="diffchange diffchange-inline">approximate </ins>guidelines about expression <ins class="diffchange diffchange-inline">strength </ins>of our vectors see Figure 1. <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">They</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h3>Key features<del class="diffchange diffchange-inline">:</h3> </del>of ExpressIt - expression vectors kit<del class="diffchange diffchange-inline">.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h3><ins class="diffchange diffchange-inline">The </ins>Key features of ExpressIt - expression vectors kit<ins class="diffchange diffchange-inline">: </h3> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">10 different measured RBS variants</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">10 different measured RBS variants</a></li></div></td></tr>
</table>Olchowikhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&diff=161573&oldid=prevOlchowik at 15:47, 26 October 20102010-10-26T15:47:04Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:47, 26 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 10:</td>
<td colspan="2" class="diff-lineno">Line 10:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><html></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ExpressIt! - family of expression vectors with defined strength</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ExpressIt! - family of expression vectors with defined strength</h2></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">For your convenience we assembled a set of expression vectors. This year we prepared two tasty flavours: J23100 base and pT7 based vectors. Those promoters are available with 10 different RBS sequences (all that we could find in the registry). For constitutive protein expression use J32100 based vectors. T7promoter is stronger than J23100, but it is active only in strains harbouring T7 polymerase. Usually expression of T7polymerase is inducible therefore pT7 is a good choice for strong inducible promoter. For approimate guidelines about expression strenght of our vectors see Figure 1. <br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h3>Key features:</h3></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">They</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h3>Key features:</h3> <ins class="diffchange diffchange-inline">of ExpressIt - expression vectors kit.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">10 different measured RBS variants</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">10 different measured RBS variants</a></li></div></td></tr>
</table>Olchowikhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&diff=151273&oldid=prevOlchowik at 23:27, 25 October 20102010-10-25T23:27:33Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:27, 25 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 20:</td>
<td colspan="2" class="diff-lineno">Line 20:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>T7 promoter is active ONLY in presence of T7 polymerase. Therefore you have to use a <i>E.Coli</i> strain containing this gene. The most popular choice <i>E.coli</i> BL21, which has a chloramphenicol resistance gene in the genome.</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>T7 promoter is active ONLY in presence of T7 polymerase. Therefore you have to use a <i>E.Coli</i> strain containing this gene. The most popular choice <i>E.coli</i> BL21, which has a chloramphenicol resistance gene in the genome.</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>terminator included in the vector sequence - pSB1A2 based.</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>terminator included in the vector sequence - <ins class="diffchange diffchange-inline"><a href="http://partsregistry.org/Part:</ins>pSB1A2<ins class="diffchange diffchange-inline">">pSB1A2</a> </ins>based.</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Olchowikhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&diff=151219&oldid=prevOlchowik at 23:23, 25 October 20102010-10-25T23:23:34Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:23, 25 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 17:</td>
<td colspan="2" class="diff-lineno">Line 17:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>just one step to clone any protein coding BioBrick and you have expression construct ready</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>just one step to clone any protein coding BioBrick and you have expression construct ready</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>wide spectrum of expression strengths</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>wide spectrum of expression strengths</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>ampicillin resistance <del class="diffchange diffchange-inline">(new variants </del>of <i>E.coli</i> BL21 <del class="diffchange diffchange-inline">are </del>chloramphenicol <del class="diffchange diffchange-inline">resistant)</del></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>ampicillin resistance <ins class="diffchange diffchange-inline">- this is important for constructs that use T7 promoter. Why? </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">T7 promoter is active ONLY in presence </ins>of <ins class="diffchange diffchange-inline">T7 polymerase. Therefore you have to use a <i>E.Coli</i> strain containing this gene. The most popular choice </ins><i>E.coli</i> BL21<ins class="diffchange diffchange-inline">, which has a </ins>chloramphenicol <ins class="diffchange diffchange-inline">resistance gene in the genome.</ins></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>terminator included in vector sequence</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>terminator included in <ins class="diffchange diffchange-inline">the </ins>vector sequence <ins class="diffchange diffchange-inline">- pSB1A2 based.</ins></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Olchowikhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&diff=124393&oldid=prevMilena igem at 12:34, 23 October 20102010-10-23T12:34:09Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 12:34, 23 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 17:</td>
<td colspan="2" class="diff-lineno">Line 17:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>just one step to clone any protein coding BioBrick and you have expression construct ready</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>just one step to clone any protein coding BioBrick and you have expression construct ready</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>wide spectrum of expression strengths</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>wide spectrum of expression strengths</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>ampicillin resistance (new variants of E. coli BL21 are chloramphenicol resistant)</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>ampicillin resistance (new variants of <ins class="diffchange diffchange-inline"><i></ins>E.coli<ins class="diffchange diffchange-inline"></i> </ins>BL21 are chloramphenicol resistant)</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>terminator included in vector sequence</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>terminator included in vector sequence</li></div></td></tr>
</table>Milena igemhttp://2010.igem.org/wiki/index.php?title=Team:Warsaw/Stage1/ExpressIt&diff=105265&oldid=prevMlower: New page: <html> <script type="text/javascript"> ActiveTab='Stage1'; </script> </html> {{TemplateTop}} {{UpperTabs}} {{LowerTabsTeam}} <html> <h2>ExpressIt! - family of expression vectors with defi...2010-10-16T23:29:51Z<p>New page: <html> <script type="text/javascript"> ActiveTab='Stage1'; </script> </html> {{TemplateTop}} {{UpperTabs}} {{LowerTabsTeam}} <html> <h2>ExpressIt! - family of expression vectors with defi...</p>
<p><b>New page</b></p><div><html><br />
<script type="text/javascript"><br />
ActiveTab='Stage1';<br />
</script><br />
</html><br />
<br />
{{TemplateTop}}<br />
{{UpperTabs}}<br />
{{LowerTabsTeam}}<br />
<html><br />
<h2>ExpressIt! - family of expression vectors with defined strength</h2><br />
<br />
<h3>Key features:</h3><br />
<ul><br />
<li><a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">10 different measured RBS variants</a></li><br />
<li>each variant in both constitutive (<a href="http://partsregistry.org/Part:BBa_J23100">J23100</a> driven) and inducible (<a href="http://partsregistry.org/Part:BBa_I719005">pT7</a> driven) version</li><br />
<li>just one step to clone any protein coding BioBrick and you have expression construct ready</li><br />
<li>wide spectrum of expression strengths</li><br />
<li>ampicillin resistance (new variants of E. coli BL21 are chloramphenicol resistant)</li><br />
<li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li><br />
<li>terminator included in vector sequence</li><br />
</ul><br />
<br />
<h3>Relative strength of ExpressIt! vector family:</h3><br />
<img src="https://static.igem.org/mediawiki/2010/0/09/ExpressIt.png"><br />
<br />
</html> <br />
<br />
{{TemplateBottom}}</div>Mlower