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Team:Warsaw/Stage1/ExpressIt
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Revision as of 23:29, 16 October 2010
ExpressIt! - family of expression vectors with defined strength
Key features:
- 10 different measured RBS variants
- each variant in both constitutive (J23100 driven) and inducible (pT7 driven) version
- just one step to clone any protein coding BioBrick and you have expression construct ready
- wide spectrum of expression strengths
- ampicillin resistance (new variants of E. coli BL21 are chloramphenicol resistant)
- high copy ColE1 ori (based on pSB1A2 vector)
- terminator included in vector sequence
Relative strength of ExpressIt! vector family:
