Team:Warsaw/Stage1/ExpressIt

From 2010.igem.org

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T7 promoter is active ONLY in presence of T7 polymerase. Therefore you have to use a <i>E.Coli</i> strain containing this gene. The most popular choice <i>E.coli</i> BL21, which has a chloramphenicol resistance gene in the genome.</li>
T7 promoter is active ONLY in presence of T7 polymerase. Therefore you have to use a <i>E.Coli</i> strain containing this gene. The most popular choice <i>E.coli</i> BL21, which has a chloramphenicol resistance gene in the genome.</li>
<li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li>
<li>high copy ColE1 ori (based on <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> vector)</li>
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<li>terminator included in the vector sequence - pSB1A2 based.</li>
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<li>terminator included in the vector sequence - <a href="http://partsregistry.org/Part:pSB1A2">pSB1A2</a> based.</li>
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</ul>

Revision as of 23:27, 25 October 2010

Example Tabs

ExpressIt! - family of expression vectors with defined strength

Key features:

  • 10 different measured RBS variants
  • each variant in both constitutive (J23100 driven) and inducible (pT7 driven) version
  • just one step to clone any protein coding BioBrick and you have expression construct ready
  • wide spectrum of expression strengths
  • ampicillin resistance - this is important for constructs that use T7 promoter. Why? T7 promoter is active ONLY in presence of T7 polymerase. Therefore you have to use a E.Coli strain containing this gene. The most popular choice E.coli BL21, which has a chloramphenicol resistance gene in the genome.
  • high copy ColE1 ori (based on pSB1A2 vector)
  • terminator included in the vector sequence - pSB1A2 based.

Relative strength of ExpressIt! vector family: