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Team:Warsaw/Project
From 2010.igem.org
Project description
Our project is divided in following parts:
- RBS Measurement
- Safety
- BactoDHL - universal platform of protein and DNA delivery to mammalian cells based on invasive coli strain
- ColiStainer - an implementation of BactoDHL which stains various compartments of alive mammalian cells using fluorescent proteins fused with localization signals
RBS Measurement
In order to fine-tune expression of genes used in our project we are conducting measurement of various ribosome binding sites included in 2010 spring distribution. Our list of measured parts includes RBSs both from Community and Anderson's collections. We are using standard measurement kit composed of promoter BBa_J23100 and GFP+terminator part BBa_I130401.
Safety
To make our invasive E. coli strain safe we are planning to use division inhibitor protein MinC from E. coli BL21. Overexpression of MinC was shown to stop bacterial divisions without impairing the rest of cell functions, such as protein synthesis.
BactoDHL
ColiStainer
