Team:Warsaw/Calendar-Stage2/21 September 2010

Revision as of 21:17, 25 October 2010 by Qba (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

• Team
• Attribution
• Sponsors
• Acknowledgemets

• Idea
• Background
• Promoters Measured
• RBSes Measured
• ExpressIt! Vectors
• Modeling

• Idea
• Background
• Design
• Results

• Idea
• Background and Design
• Results
• Gallery of microphotographs

• Idea
• Background
• Design
• Results
• Modeling

• Parts
• Labbook

• Licensing of biological parts
• Syntetic biology in Poland
• Synthetic BioLectures gallery
• iGEM survey

• About Biobrick Manager
• See Biobrick Manager

Purification of MinC expression vectors

Kuba

Six colonies were picked for inoculation of liquid cultures (three with B0032 and three with B0034). Cultures were grown for 6 h in 37 ^oC. Plasmids were purified with Plasmid Mini kit (A&A) biotechnology. The obtained sample, as well as the initial (unmutated) plasmid, were then digested with EcoRI and PstI for 1 h and run on agarose gel.

M - size marker; 32a,b,c - plasmids harboring MinC under weak RBS; 34a,b,c - plasmids harboring MinC under strong RBS

Samples 32a,b&c ans well as 34b&c appear to contain a correct construct. Smaller amounts of DNA with B0034-containing constructs may indicate a slight leaking of the construct (probably due to a read-through from the resistance gene).