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Team:Warsaw/Calendar-Stage2/21 September 2010
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<p align="justify">Six colonies were picked for inoculation of liquid cultures (three with B0032 and three with B0034). Cultures were grown for 6 h in 37 <sup>o</sup>C. Plasmids were purified with Plasmid Mini kit (A&A) biotechnology. The obtained sample, as well as the initial (unmutated) plasmid, were then digested with EcoRI and PstI for 1 h and run on agarose gel.</p> | <p align="justify">Six colonies were picked for inoculation of liquid cultures (three with B0032 and three with B0034). Cultures were grown for 6 h in 37 <sup>o</sup>C. Plasmids were purified with Plasmid Mini kit (A&A) biotechnology. The obtained sample, as well as the initial (unmutated) plasmid, were then digested with EcoRI and PstI for 1 h and run on agarose gel.</p> | ||
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| - | <img src="https://static.igem.org/mediawiki/2010/e/e3/Minc1.jpg" height=400 width= | + | <img src="https://static.igem.org/mediawiki/2010/e/e3/Minc1.jpg" height=400 width=500> |
<p align="left"><i> M - size marker; 32a,b,c - plasmids harboring MinC under weak RBS; 34a,b,c - plasmids harboring MinC under strong RBS</i></p> | <p align="left"><i> M - size marker; 32a,b,c - plasmids harboring MinC under weak RBS; 34a,b,c - plasmids harboring MinC under strong RBS</i></p> | ||
<br> | <br> | ||
| - | <p align="justify">Samples 32a,b&c ans well as 34b&c appear to contain a correct construct. Smaller amounts of DNA | + | <p align="justify">Samples 32a,b&c ans well as 34b&c appear to contain a correct construct. Smaller amounts of DNA with B0034-containing constructs may indicate a slight leaking of the construct (probably due to a read-through from the resistance gene).</p> |
Latest revision as of 21:17, 25 October 2010
Purification of MinC expression vectors
Kuba
Six colonies were picked for inoculation of liquid cultures (three with B0032 and three with B0034). Cultures were grown for 6 h in 37 oC. Plasmids were purified with Plasmid Mini kit (A&A) biotechnology. The obtained sample, as well as the initial (unmutated) plasmid, were then digested with EcoRI and PstI for 1 h and run on agarose gel.
M - size marker; 32a,b,c - plasmids harboring MinC under weak RBS; 34a,b,c - plasmids harboring MinC under strong RBS
Samples 32a,b&c ans well as 34b&c appear to contain a correct construct. Smaller amounts of DNA with B0034-containing constructs may indicate a slight leaking of the construct (probably due to a read-through from the resistance gene).
