Team:Warsaw/Calendar-Stage2/17 September 2010

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• Team
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• Idea
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Mutagenesis of MinC

Kuba

Four colonies were picked for inoculation of liquid cultures. Cultures were grown for 6 h in 37 ^oC. Plasmids were purified with Plasmid Mini kit (A&A) biotechnology. The obtained sample, as well as the initial (unmutated) plasmid, were then digested with EcoRI and PstI for 1 h and run on agarose gel.
C - unmutated plasmid; M - marker; 1,2,3,4 -respective samples
The shorter band in samples 1,2,3 appears to be slightly longer compared to control, indicating a succesfull mutagenesis. These conclusions were later verified by sequencing.