Team:Warsaw/Calendar-Stage1/8 September 2010

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<br> 07.09.2010 Tuesday
 
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<br><div class="note"> Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]</div>
 
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<br>1. Enclosing GFP in different pH buffers into droplets in  a water-in-oil-in-water emulsion
 
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<br>2. Preparation of probes and examining them in flow cytometer
 
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<br><div class="note"> Data analysis with Cell Quest[Cherry]</div>
 
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<br /><strong>legend:</strong>
 
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<hr />
 
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<br />07-09-2010.004- pH 7
 
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<br />07-09-2010.005- pH 8
 
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<br />07-09-2010.006- pH 6,8
 
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<br />07-09-2010.007- pH 4,8
 
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<br />07-09-2010.010- pH 8
 
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Data from Cell Quest analysis:
 
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<img src="http://2010.igem.org/wiki/images/d/db/Rbs.7.09_01.png" width="600px" />
 
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<img src="http://2010.igem.org/wiki/images/8/8b/Rbs.7.09_02.png" width="600px" />
 
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<img src="http://2010.igem.org/wiki/images/c/cf/Rbs.7.09_03.png" width="600px" />
 
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<img src="http://2010.igem.org/wiki/images/c/c3/Rbs.7.09_04.png" width="600px" />
 
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<img src="http://2010.igem.org/wiki/images/4/4d/Rbs.7.09_05.png" width="600px" />
 
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<img src="http://2010.igem.org/wiki/images/6/6c/Rbs.7.09_06.png" width="600px" />
 
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Latest revision as of 22:57, 27 October 2010

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