Team:Warsaw/Calendar-Stage1/8 September 2010
From 2010.igem.org
(Difference between revisions)
Line 15: | Line 15: | ||
<br> | <br> | ||
- | <br><div class="note"> Testing the fluorescence of GFP in different pH in a | + | <br><div class="note"> Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]</div> |
- | <br>1. | + | <br>1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion |
<br>2. Preparation of probes and examining them in flow cytometer | <br>2. Preparation of probes and examining them in flow cytometer | ||
<br> | <br> |
Revision as of 15:12, 27 October 2010
07.09.2010 Tuesday
Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]
1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion
2. Preparation of probes and examining them in flow cytometer
Data analysis with Cell Quest[Cherry]
legend:
07-09-2010.004- pH 7
07-09-2010.005- pH 8
07-09-2010.006- pH 6,8
07-09-2010.007- pH 4,8
07-09-2010.010- pH 8
Data from Cell Quest analysis: