Team:Warsaw/Calendar-Stage1/8 September 2010

From 2010.igem.org

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<br><div class="note"> Testing the fluorescence of GFP in different pH in a non-cellular system [Ania O.,Cherry]</div>
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<br><div class="note"> Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]</div>
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<br>1. Use protocol...
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<br>1. Enclosing GFP in different pH buffers into droplets in  a water-in-oil-in-water emulsion
<br>2. Preparation of probes and examining them in flow cytometer
<br>2. Preparation of probes and examining them in flow cytometer
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Revision as of 15:12, 27 October 2010

Example Tabs


07.09.2010 Tuesday

Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]

1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion
2. Preparation of probes and examining them in flow cytometer

Data analysis with Cell Quest[Cherry]


legend:

07-09-2010.004- pH 7
07-09-2010.005- pH 8
07-09-2010.006- pH 6,8
07-09-2010.007- pH 4,8
07-09-2010.010- pH 8



Data from Cell Quest analysis: