Team:Warsaw/Calendar-Stage1/7 September 2010

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<br> 07.09.2010 Tuesday
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<br>
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<br><div class="note"> Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]</div>
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<br>1. Enclosing GFP in different pH buffers into droplets in  a water-in-oil-in-water emulsion
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<br>2. Preparation of samples and examining them in flow cytometer
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<br>
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<br><div class="note"> Data analysis with Cell Quest[Cherry]</div>
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<br>
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<br /><strong>legend:</strong>
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<hr />
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<br />07-09-2010.004- pH 7
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<br />07-09-2010.005- pH 8
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<br />07-09-2010.006- pH 6,8
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<br />07-09-2010.007- pH 4,8
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<br />07-09-2010.010- pH 8
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<br /><br />cytometer plots:
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<br>FSC- Forward Scatter, cell size
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<br>SSC- Side Scatter, cell complexity
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<br>FL-1- FL-1 sensor, cell fluorescence
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<br /><br><hr />
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<br />
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Data from Cell Quest analysis:
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<br />
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<img src="https://static.igem.org/mediawiki/2010/d/db/Rbs.7.09_01.png" width="600px" />
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<img src="https://static.igem.org/mediawiki/2010/8/8b/Rbs.7.09_02.png" width="600px" />
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<img src="https://static.igem.org/mediawiki/2010/c/cf/Rbs.7.09_03.png" width="600px" />
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<img src="https://static.igem.org/mediawiki/2010/c/c3/Rbs.7.09_04.png" width="600px" />
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<img src="https://static.igem.org/mediawiki/2010/4/4d/Rbs.7.09_05.png" width="600px" />
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<img src="https://static.igem.org/mediawiki/2010/6/6c/Rbs.7.09_06.png" width="600px" />
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Latest revision as of 21:58, 27 October 2010

Example Tabs


07.09.2010 Tuesday

Testing the fluorescence of GFP in different pH in a microfluidic systemm by using flow cytometry[Ania O.,Cherry]

1. Enclosing GFP in different pH buffers into droplets in a water-in-oil-in-water emulsion
2. Preparation of samples and examining them in flow cytometer

Data analysis with Cell Quest[Cherry]


legend:

07-09-2010.004- pH 7
07-09-2010.005- pH 8
07-09-2010.006- pH 6,8
07-09-2010.007- pH 4,8
07-09-2010.010- pH 8

cytometer plots:
FSC- Forward Scatter, cell size
SSC- Side Scatter, cell complexity
FL-1- FL-1 sensor, cell fluorescence


Data from Cell Quest analysis:




Retrieved from "http://2010.igem.org/Team:Warsaw/Calendar-Stage1/7_September_2010"