Team:Warsaw/Calendar-Stage1/30 August 2010

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Example Tabs


30.08.2010 Monday

Previous day: Set up liquid cultures of E.coli with GFP with different RBS [Ania P.]

Use a protocol from here

Testing the strenght of RBS [Ania P.,Cherry]

Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer

Data analysis with Cell Quest and Microsoft Excel[Cherry]


legend:

30-08-2010.001- TOP 10
30-08-2010.007- B0030
30-08-2010.008- B0031
30-08-2010.009- B0032
30-08-2010.010- B0033
30-08-2010.011- B0034
30-08-2010.012- J61100
30-08-2010.013- J61101
30-08-2010.014- J61107
30-08-2010.015- J61117
30-08-2010.016- J61127





Cytometer data processed with Cell Quest:




Cell Quest data processed with Microsoft Excel:



geo mean- geometric mean of fluorescence of events in FL1
peak channel- nr of channel which recorded the biggest nr of events


partgeo meanpeak channel
allm1allm1
J6110022,1142,66154
J6110195,38362,431406
J6110761,84209,271228
J6111757,75171,531212
J6112721,3152,81152
B0030187,45913,8511074
B0031190,21907,6211154
B0032103,05406,11378
B00336,199,9111
B0034182,7999511333
partgeo mean %B0033peak channel %B0033
allm1allm1
J6110012,10%4,29%100,00%4,05%
J6110152,18%36,43%100,00%30,46%
J6110733,83%21,03%100,00%17,10%
J6111731,59%17,24%100,00%15,90%
J6112711,66%5,31%100,00%3,90%
B0030102,55%91,84%100,00%80,57%
B0031104,06%91,22%100,00%86,57%
B003256,38%40,81%100,00%28,36%
B00333,39%0,99%100,00%0,83%
B0034100,00%100,00%100,00%100,00%