Team:Warsaw/Calendar-Stage1/30 August 2010
From 2010.igem.org
30.08.2010 Monday
Previous day: Set up liquid cultures of E.coli with GFP with different RBS [Ania P.]
Use a protocol from here
Testing the strenght of RBS [Ania P.,Cherry]
Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer
Data analysis with Cell Quest and Microsoft Excel[Cherry]
legend:
30-08-2010.001- TOP 10
30-08-2010.007- B0030
30-08-2010.008- B0031
30-08-2010.009- B0032
30-08-2010.010- B0033
30-08-2010.011- B0034
30-08-2010.012- J61100
30-08-2010.013- J61101
30-08-2010.014- J61107
30-08-2010.015- J61117
30-08-2010.016- J61127
Cytometer data processed with Cell Quest:
Cell Quest data processed with Microsoft Excel:
geo mean- geometric mean of fluorescence of events in FL1
peak channel- nr of channel which recorded the biggest nr of events
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