Team:Warsaw/Calendar-Stage1/29 August 2010
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<br />Cytometer data from Cell Quest analysis: | <br />Cytometer data from Cell Quest analysis: | ||
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<img src="https://static.igem.org/mediawiki/2010/e/ec/Bacto.30.08_03.png" width="500px" /> | <img src="https://static.igem.org/mediawiki/2010/e/ec/Bacto.30.08_03.png" width="500px" /> |
Revision as of 20:58, 26 October 2010
30.08.2010 Monday
Previous 2 days: Set up and culturing Hela cell culture [Kamil]
Use a protocol...
Previous day: Set up liquid cultures of different types of E.coli [Marta B.]
Use a protocol...
Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]
1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells (using lysozyme and Triton) and examining them in flow cytometer
Data analysis with Cell Quest and Microsoft Excel [Cherry]
legend:
E.coli constructs:
G- GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP
TOP- without construct
LIZ- treated with lysozyme
TRIT- treated with triton
X- untreated
bacteria:
30-08-2010.001- TOP
30-08-2010.003- G
30-08-2010.004- J
30-08-2010.005- A
Hela cells with bacteria:
30-08-2010.033- X TRIT
30-08-2010.034- A TRIT
30-08-2010.035- J TRIT
30-08-2010.036- A LIZ
30-08-2010.037- J LIZ
30-08-2010.038- without bacteria TRIT
30-08-2010.039- TOP TRIT
30-08-2010.040- G TRIT
30-08-2010.041- X LIZ
30-08-2010.042- without bacteria LIZ
30-08-2010.043- TOP LIZ
30-08-2010.044- G LIZ
Cytometer data from Cell Quest analysis:
Data from Cell Quest processed with Microsoft Excel: