Team:Warsaw/Calendar-Stage1/24 July 2010

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<br> 23.07.2010 Friday
 
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<br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div>
 
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<br> Use a protocol...
 
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<br><div class="note"> Previous day: Set up liquid cultures of different types of E.coli [Ania P.]</div>
 
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<br> Use a protocol...
 
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<br><div class="note"> Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]</div>
 
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<br>1. Incubation of Hela cells with different E.coli types
 
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<br>2. Preparation of probes with incubated cells and examining them in flow cytometer
 
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<br><div class="note"> Data analysis with Cell Quest and Microsoft Excel [Cherry]</div>
 
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<br /><strong>legend</strong>:
 
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<hr />
 
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<br /><strong>E.coli constructs:</strong>
 
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<br />G- GFP
 
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<br />I- Invasin+GFP
 
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<br />J- JPH+Listeriolysin O+Invasin+GFP
 
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<br />A- APH+Listeriolysin O+Invasin+GFP
 
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<br /><strong>Hela cells with bacteria:</strong>
 
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<br />23-07-2010.007- without bacteria
 
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<br />23-07-2010.018- with G (1)
 
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<br />23-07-2010.019- with G (2)
 
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<br />23-07-2010.020- with G (3)
 
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<br />23-07-2010.015- with I (1)
 
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<br />23-07-2010.016- with I (2)
 
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<br />23-07-2010.017- with I (3)
 
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<br />23-07-2010.012- with J (1)
 
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<br />23-07-2010.013- with J (2)
 
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<br />23-07-2010.014- with J (3)
 
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<br />23-07-2010.008- with A (1)
 
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<br />23-07-2010.010- with A (2)
 
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<br />23-07-2010.011- with A (3)
 
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<br /><strong>Data processed with Cell Quest: </strong><br/>
 
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<br><br/><img src="https://static.igem.org/mediawiki/2010/d/da/RBS.23.07_01.png" width="450px" />
 
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<img src="https://static.igem.org/mediawiki/2010/9/94/RBS.23.07_02.png" width="750px" />
 
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<img src="https://static.igem.org/mediawiki/2010/5/55/RBS.23.07_03.png" width="750px" />
 
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<img src="https://static.igem.org/mediawiki/2010/7/7f/RBS.23.07_04.png" width="750px" />
 
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<img src="https://static.igem.org/mediawiki/2010/d/d4/RBS.23.07_05.png" width="750px" />
 
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<img src="https://static.igem.org/mediawiki/2010/1/12/RBS.23.07_06.png" width="750px" />
 
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<hr /><br>
 
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<strong>Data from Cell Quest processed with Microsoft Excel:</strong>
 
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<br><br/><br/>
 
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legend:
 
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<br>Hela N- without bacteria
 
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<br>M1,M2,M3,M4- %of events gated with markers M from total
 
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<br>SD- starndard deviation
 
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<br>Err.-errror
 
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<img src="https://static.igem.org/mediawiki/2010/a/a8/Bacto.23.07_06_table1.png" width="800px" />
 
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<br />more exact data:
 
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<img src="https://static.igem.org/mediawiki/2010/3/34/Bacto.23.07_06_table2.png" width="925px" />
 
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Latest revision as of 22:55, 27 October 2010

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