Team:Warsaw/Calendar-Stage1/24 July 2010

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(Difference between revisions)

Revision as of 22:17, 26 October 2010

Example Tabs

23.07.2010 Friday

Note: We are not totally satisfied with the results, because of the performance of the cells during the flow cytometry examination, they where adhering to each other.

Previous 2 days: Set up and culturing Hela cell culture [Kamil]

Use a protocol...

Previous day: Set up liquid cultures of different types of E.coli [Ania P.]

Use a protocol...

Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]

1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells and examining them in flow cytometer

Data analysis with Cell Quest and Microsoft Excel [Cherry]

legend:

E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP

Hela cells with bacteria:
23-07-2010.007- without bacteria
23-07-2010.018- with G (1)
23-07-2010.019- with G (2)
23-07-2010.020- with G (3)
23-07-2010.015- with I (1)
23-07-2010.016- with I (2)
23-07-2010.017- with I (3)
23-07-2010.012- with J (1)
23-07-2010.013- with J (2)
23-07-2010.014- with J (3)
23-07-2010.008- with A (1)
23-07-2010.010- with A (2)
23-07-2010.011- with A (3)

Data processed with Cell Quest:

Data from Cell Quest processed with Microsoft Excel:

legend:
Hela N- without bacteria
M1,M2,M3,M4- %of events gated with markers M from total
SD- starndard deviation
ERR.-errror

more exact data:

@@ Line 12: / Line 12: @@
 <br> 23.07.2010 Friday
-<br>Note: We are not totally satisfied with the results, because of the performance of the cells during the flow cytometry examination, they where adhering to each other.
+<br><br>Note: We are not totally satisfied with the results, because of the performance of the cells during the flow cytometry examination, they where adhering to each other.
 <br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div>
 <br> Use a protocol...