Team:Warsaw/Calendar-Stage1/24 July 2010

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Revision as of 19:35, 26 October 2010

Example Tabs


23.07.2010 Friday

Previous 2 days: Set up and culturing Hela cell culture [Kamil]

Use a protocol...

Previous day: Set up liquid cultures of different types of E.coli [Ania P.]

Use a protocol...

Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]

1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells and examining them in flow cytometer

Data analysis with Cell Quest and Microsoft Excel [Cherry]


legend:

E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP

Hela cells with bacteria:
23-07-2010.007- without bacteria
23-07-2010.018- with G (1)
23-07-2010.019- with G (2)
23-07-2010.020- with G (3)
23-07-2010.015- with I (1)
23-07-2010.016- with I (2)
23-07-2010.017- with I (3)
23-07-2010.012- with J (1)
23-07-2010.013- with J (2)
23-07-2010.014- with J (3)
23-07-2010.008- with A (1)
23-07-2010.010- with A (2)
23-07-2010.011- with A (3)




Data processed with Cell Quest:







Data from Cell Quest processed with Microsoft Excel: