Team:Warsaw/Calendar-Stage1/24 July 2010
From 2010.igem.org
(Difference between revisions)
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</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
+ | <br /> | ||
+ | <br /> | ||
+ | <br /> | ||
+ | <br /> | ||
+ | |||
+ | <table x:str border="0" style="border-collapse: collapse"><tr> | ||
+ | <td> | ||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td></td><td>Hela N</td></tr> | ||
+ | <tr><td>M1</td><td>86,66</td></tr> | ||
+ | <tr><td>M2</td><td>64,93</td></tr> | ||
+ | <tr><td>M3</td><td>3,3</td></tr> | ||
+ | <tr><td>M4</td><td>96,59</td></tr> | ||
+ | </table> | ||
+ | </td><td> | ||
+ | |||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td>g1</td><td>g2</td><td>g3</td></tr> | ||
+ | <tr><td>32,19</td><td>34,27</td><td>30,73</td></tr> | ||
+ | <tr><td>47,4</td><td>53</td><td>43</td></tr> | ||
+ | <tr><td>28,09</td><td>26,24</td><td>29,2</td></tr> | ||
+ | <tr><td>50,63</td><td>55,4</td><td>47,16</td></tr> | ||
+ | </table> | ||
+ | </td><td> | ||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr> | ||
+ | <tr><td>32,4</td><td>1,78</td><td>5,49</td></tr> | ||
+ | <tr><td>47,8</td><td>5,01</td><td>10,49</td></tr> | ||
+ | <tr><td>27,84</td><td>1,5</td><td>5,37</td></tr> | ||
+ | <tr><td>51,06</td><td>4,14</td><td>8,1</td></tr> | ||
+ | </table> | ||
+ | </td><td> | ||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td>i1</td><td>i2</td><td>i3</td></tr> | ||
+ | <tr><td>35,06</td><td>37,85</td><td>39,18</td></tr> | ||
+ | <tr><td>48,66</td><td>55,19</td><td>56,39</td></tr> | ||
+ | <tr><td>26,45</td><td>25,26</td><td>23,72</td></tr> | ||
+ | <tr><td>53,41</td><td>59,48</td><td>60,46</td></tr> | ||
+ | </table> | ||
+ | </td><td> | ||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr> | ||
+ | <tr><td>37,36</td><td>2,1</td><td>5,63</td></tr> | ||
+ | <tr><td>53,41</td><td>4,16</td><td>7,79</td></tr> | ||
+ | <tr><td>25,14</td><td>1,37</td><td>5,44</td></tr> | ||
+ | <tr><td>57,78</td><td>3,82</td><td>6,61</td></tr> | ||
+ | </table> | ||
+ | </td> | ||
+ | </tr></table> | ||
+ | <br /> | ||
+ | <br /> | ||
+ | <table border="0" style="border-collapse: collapse"><tr> | ||
+ | <td> | ||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td> </td></tr> | ||
+ | <tr><td>M1</td></tr> | ||
+ | <tr><td>M2</td></tr> | ||
+ | <tr><td>M3</td></tr> | ||
+ | <tr><td>M4</td></tr> | ||
+ | </table> | ||
+ | </td><td> | ||
+ | |||
+ | |||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td>j1</td><td>j2</td><td>j3</td></tr> | ||
+ | <tr><td>8,01</td><td>16,03</td><td>8,49</td></tr> | ||
+ | <tr><td>28,51</td><td>39,35</td><td>29,22</td></tr> | ||
+ | <tr><td>59,24</td><td>52,3</td><td>61,5</td></tr> | ||
+ | <tr><td>24,15</td><td>37,66</td><td>24,79</td></tr> | ||
+ | </table> | ||
+ | </td><td> | ||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr> | ||
+ | <tr><td>10,84</td><td>4,5</td><td>41,48</td></tr> | ||
+ | <tr><td>32,36</td><td>6,06</td><td>18,74</td></tr> | ||
+ | <tr><td>57,68</td><td>4,79</td><td>8,31</td></tr> | ||
+ | <tr><td>28,87</td><td>7,62</td><td>26,4</td></tr> | ||
+ | </table> | ||
+ | </td><td> | ||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td>a1</td><td>a2</td><td>a3</td></tr> | ||
+ | <tr><td>18,78</td><td>10,07</td><td>8,84</td></tr> | ||
+ | <tr><td>46,45</td><td>29,68</td><td>30,58</td></tr> | ||
+ | <tr><td>43,15</td><td>53,05</td><td>56,19</td></tr> | ||
+ | <tr><td>44,29</td><td>26,48</td><td>26,31</td></tr> | ||
+ | </table> | ||
+ | </td><td> | ||
+ | <table border="1" style="border-collapse: collapse" cellpading="15px"> | ||
+ | <tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr> | ||
+ | <tr><td>12,56</td><td>5,42</td><td>43,13</td></tr> | ||
+ | <tr><td>35,57</td><td>9,43</td><td>26,52</td></tr> | ||
+ | <tr><td>50,8</td><td>6,81</td><td>13,4</td></tr> | ||
+ | <tr><td>32,36</td><td>10,33</td><td>31,93</td></tr> | ||
+ | </table> | ||
+ | </td> | ||
+ | </tr></table> | ||
+ | |||
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<br /> | <br /> |
Revision as of 18:56, 26 October 2010
23.07.2010 Friday
Previous 2 days: Set up and culturing Hela cell culture [Kamil]
Use a protocol...
Previous day: Set up liquid cultures of different types of E.coli [Ania P.]
Use a protocol...
Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]
1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells and examining them in flow cytometer
Data analysis with Cell Quest and Microsoft Excel [Cherry]
legend:
E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP
Hela cells with bacteria:
23-07-2010.007- without bacteria
23-07-2010.018- with G (1)
23-07-2010.019- with G (2)
23-07-2010.020- with G (3)
23-07-2010.015- with I (1)
23-07-2010.016- with I (2)
23-07-2010.017- with I (3)
23-07-2010.012- with J (1)
23-07-2010.013- with J (2)
23-07-2010.014- with J (3)
23-07-2010.008- with A (1)
23-07-2010.010- with A (2)
23-07-2010.011- with A (3)
Data processed with Cell Quest:
Data from Cell Quest processed with Microsoft Excel:
Hela N | Hela G | Hela I | Hela J | Hela A | |||||||||
MEAN | SD | bląd | MEAN | SD | ERR. | MEAN | SD | ERR. | MEAN | SD | ERR. | ||
M1 | 86,66 | 32,40 | 1,78 | 5,49 | 37,36 | 2,10 | 5,63 | 10,84 | 4,50 | 41,48 | 12,56 | 5,42 | 43,13 |
M2 | 64,93 | 47,80 | 5,01 | 10,49 | 53,41 | 4,16 | 7,79 | 32,36 | 6,06 | 18,74 | 35,57 | 9,43 | 26,52 |
M3 | 3,30 | 27,84 | 1,50 | 5,37 | 25,14 | 1,37 | 5,44 | 57,68 | 4,79 | 8,31 | 50,80 | 6,81 | 13,40 |
M4 | 96,59 | 51,06 | 4,14 | 8,10 | 57,78 | 3,82 | 6,61 | 28,87 | 7,62 | 26,40 | 32,36 | 10,33 | 31,93 |
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