Team:Warsaw/Calendar-Stage1/24 July 2010

From 2010.igem.org

(Difference between revisions)
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<table x:str border="0" style="border-collapse: collapse"><tr>
 
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<td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td></td><td>Hela N</td></tr>
 
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<tr><td>M1</td><td>86,66</td></tr>
 
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<tr><td>M2</td><td>64,93</td></tr>
 
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<tr><td>M3</td><td>3,3</td></tr>
 
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<tr><td>M4</td><td>96,59</td></tr>
 
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</table>
 
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</td><td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td>g1</td><td>g2</td><td>g3</td></tr>
 
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<tr><td>32,19</td><td>34,27</td><td>30,73</td></tr>
 
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<tr><td>47,4</td><td>53</td><td>43</td></tr>
 
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<tr><td>28,09</td><td>26,24</td><td>29,2</td></tr>
 
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<tr><td>50,63</td><td>55,4</td><td>47,16</td></tr>
 
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</table>
 
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</td><td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr>
 
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<tr><td>32,4</td><td>1,78</td><td>5,49</td></tr>
 
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<tr><td>47,8</td><td>5,01</td><td>10,49</td></tr>
 
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<tr><td>27,84</td><td>1,5</td><td>5,37</td></tr>
 
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<tr><td>51,06</td><td>4,14</td><td>8,1</td></tr>
 
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</table>
 
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</td><td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td>i1</td><td>i2</td><td>i3</td></tr>
 
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<tr><td>35,06</td><td>37,85</td><td>39,18</td></tr>
 
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<tr><td>48,66</td><td>55,19</td><td>56,39</td></tr>
 
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<tr><td>26,45</td><td>25,26</td><td>23,72</td></tr>
 
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<tr><td>53,41</td><td>59,48</td><td>60,46</td></tr>
 
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</table>
 
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</td><td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr>
 
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<tr><td>37,36</td><td>2,1</td><td>5,63</td></tr>
 
-
<tr><td>53,41</td><td>4,16</td><td>7,79</td></tr>
 
-
<tr><td>25,14</td><td>1,37</td><td>5,44</td></tr>
 
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<tr><td>57,78</td><td>3,82</td><td>6,61</td></tr>
 
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</table>
 
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</td>
 
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</tr></table>
 
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<br />
 
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<br />
 
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<table border="0" style="border-collapse: collapse"><tr>
 
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<td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td>&nbsp;</td></tr>
 
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<tr><td>M1</td></tr>
 
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<tr><td>M2</td></tr>
 
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<tr><td>M3</td></tr>
 
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<tr><td>M4</td></tr>
 
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</table>
 
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</td><td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td>j1</td><td>j2</td><td>j3</td></tr>
 
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<tr><td>8,01</td><td>16,03</td><td>8,49</td></tr>
 
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<tr><td>28,51</td><td>39,35</td><td>29,22</td></tr>
 
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<tr><td>59,24</td><td>52,3</td><td>61,5</td></tr>
 
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<tr><td>24,15</td><td>37,66</td><td>24,79</td></tr>
 
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</table>
 
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</td><td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr>
 
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<tr><td>10,84</td><td>4,5</td><td>41,48</td></tr>
 
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<tr><td>32,36</td><td>6,06</td><td>18,74</td></tr>
 
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<tr><td>57,68</td><td>4,79</td><td>8,31</td></tr>
 
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<tr><td>28,87</td><td>7,62</td><td>26,4</td></tr>
 
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</table>
 
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</td><td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td>a1</td><td>a2</td><td>a3</td></tr>
 
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<tr><td>18,78</td><td>10,07</td><td>8,84</td></tr>
 
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<tr><td>46,45</td><td>29,68</td><td>30,58</td></tr>
 
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<tr><td>43,15</td><td>53,05</td><td>56,19</td></tr>
 
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<tr><td>44,29</td><td>26,48</td><td>26,31</td></tr>
 
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</table>
 
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</td><td>
 
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<table border="1" style="border-collapse: collapse" cellpading="15px">
 
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<tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr>
 
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<tr><td>12,56</td><td>5,42</td><td>43,13</td></tr>
 
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<tr><td>35,57</td><td>9,43</td><td>26,52</td></tr>
 
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<tr><td>50,8</td><td>6,81</td><td>13,4</td></tr>
 
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<tr><td>32,36</td><td>10,33</td><td>31,93</td></tr>
 
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</table>
 
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</td>
 
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</tr></table>
 
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<br />
 
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   </tr>
   </tr>
</table>
</table>
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<br />
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<br />
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<br />
 +
<br />
 +
 +
<table x:str border="0" style="border-collapse: collapse"><tr>
 +
<td>
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td></td><td>Hela N</td></tr>
 +
<tr><td>M1</td><td>86,66</td></tr>
 +
<tr><td>M2</td><td>64,93</td></tr>
 +
<tr><td>M3</td><td>3,3</td></tr>
 +
<tr><td>M4</td><td>96,59</td></tr>
 +
</table>
 +
</td><td>
 +
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td>g1</td><td>g2</td><td>g3</td></tr>
 +
<tr><td>32,19</td><td>34,27</td><td>30,73</td></tr>
 +
<tr><td>47,4</td><td>53</td><td>43</td></tr>
 +
<tr><td>28,09</td><td>26,24</td><td>29,2</td></tr>
 +
<tr><td>50,63</td><td>55,4</td><td>47,16</td></tr>
 +
</table>
 +
</td><td>
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr>
 +
<tr><td>32,4</td><td>1,78</td><td>5,49</td></tr>
 +
<tr><td>47,8</td><td>5,01</td><td>10,49</td></tr>
 +
<tr><td>27,84</td><td>1,5</td><td>5,37</td></tr>
 +
<tr><td>51,06</td><td>4,14</td><td>8,1</td></tr>
 +
</table>
 +
</td><td>
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td>i1</td><td>i2</td><td>i3</td></tr>
 +
<tr><td>35,06</td><td>37,85</td><td>39,18</td></tr>
 +
<tr><td>48,66</td><td>55,19</td><td>56,39</td></tr>
 +
<tr><td>26,45</td><td>25,26</td><td>23,72</td></tr>
 +
<tr><td>53,41</td><td>59,48</td><td>60,46</td></tr>
 +
</table>
 +
</td><td>
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr>
 +
<tr><td>37,36</td><td>2,1</td><td>5,63</td></tr>
 +
<tr><td>53,41</td><td>4,16</td><td>7,79</td></tr>
 +
<tr><td>25,14</td><td>1,37</td><td>5,44</td></tr>
 +
<tr><td>57,78</td><td>3,82</td><td>6,61</td></tr>
 +
</table>
 +
</td>
 +
</tr></table>
 +
<br />
 +
<br />
 +
<table border="0" style="border-collapse: collapse"><tr>
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<td>
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td>&nbsp;</td></tr>
 +
<tr><td>M1</td></tr>
 +
<tr><td>M2</td></tr>
 +
<tr><td>M3</td></tr>
 +
<tr><td>M4</td></tr>
 +
</table>
 +
</td><td>
 +
 +
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td>j1</td><td>j2</td><td>j3</td></tr>
 +
<tr><td>8,01</td><td>16,03</td><td>8,49</td></tr>
 +
<tr><td>28,51</td><td>39,35</td><td>29,22</td></tr>
 +
<tr><td>59,24</td><td>52,3</td><td>61,5</td></tr>
 +
<tr><td>24,15</td><td>37,66</td><td>24,79</td></tr>
 +
</table>
 +
</td><td>
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr>
 +
<tr><td>10,84</td><td>4,5</td><td>41,48</td></tr>
 +
<tr><td>32,36</td><td>6,06</td><td>18,74</td></tr>
 +
<tr><td>57,68</td><td>4,79</td><td>8,31</td></tr>
 +
<tr><td>28,87</td><td>7,62</td><td>26,4</td></tr>
 +
</table>
 +
</td><td>
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td>a1</td><td>a2</td><td>a3</td></tr>
 +
<tr><td>18,78</td><td>10,07</td><td>8,84</td></tr>
 +
<tr><td>46,45</td><td>29,68</td><td>30,58</td></tr>
 +
<tr><td>43,15</td><td>53,05</td><td>56,19</td></tr>
 +
<tr><td>44,29</td><td>26,48</td><td>26,31</td></tr>
 +
</table>
 +
</td><td>
 +
<table border="1" style="border-collapse: collapse" cellpading="15px">
 +
<tr><td>MEAN</td><td>SD</td><td>ERR.</td></tr>
 +
<tr><td>12,56</td><td>5,42</td><td>43,13</td></tr>
 +
<tr><td>35,57</td><td>9,43</td><td>26,52</td></tr>
 +
<tr><td>50,8</td><td>6,81</td><td>13,4</td></tr>
 +
<tr><td>32,36</td><td>10,33</td><td>31,93</td></tr>
 +
</table>
 +
</td>
 +
</tr></table>
 +
 +
 +
<br />
<br />

Revision as of 18:56, 26 October 2010

Example Tabs


23.07.2010 Friday

Previous 2 days: Set up and culturing Hela cell culture [Kamil]

Use a protocol...

Previous day: Set up liquid cultures of different types of E.coli [Ania P.]

Use a protocol...

Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]

1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells and examining them in flow cytometer

Data analysis with Cell Quest and Microsoft Excel [Cherry]


legend:

E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP

Hela cells with bacteria:
23-07-2010.007- without bacteria
23-07-2010.018- with G (1)
23-07-2010.019- with G (2)
23-07-2010.020- with G (3)
23-07-2010.015- with I (1)
23-07-2010.016- with I (2)
23-07-2010.017- with I (3)
23-07-2010.012- with J (1)
23-07-2010.013- with J (2)
23-07-2010.014- with J (3)
23-07-2010.008- with A (1)
23-07-2010.010- with A (2)
23-07-2010.011- with A (3)




Data processed with Cell Quest:







Data from Cell Quest processed with Microsoft Excel:



  Hela N Hela G     Hela I     Hela J     Hela A    
    MEAN SD bląd MEAN SD ERR. MEAN SD ERR. MEAN SD ERR.
M1 86,66 32,40 1,78 5,49 37,36 2,10 5,63 10,84 4,50 41,48 12,56 5,42 43,13
M2 64,93 47,80 5,01 10,49 53,41 4,16 7,79 32,36 6,06 18,74 35,57 9,43 26,52
M3 3,30 27,84 1,50 5,37 25,14 1,37 5,44 57,68 4,79 8,31 50,80 6,81 13,40
M4 96,59 51,06 4,14 8,10 57,78 3,82 6,61 28,87 7,62 26,40 32,36 10,33 31,93




Hela N
M186,66
M264,93
M33,3
M496,59
g1g2g3
32,1934,2730,73
47,45343
28,0926,2429,2
50,6355,447,16
MEANSDERR.
32,41,785,49
47,85,0110,49
27,841,55,37
51,064,148,1
i1i2i3
35,0637,8539,18
48,6655,1956,39
26,4525,2623,72
53,4159,4860,46
MEANSDERR.
37,362,15,63
53,414,167,79
25,141,375,44
57,783,826,61


 
M1
M2
M3
M4
j1j2j3
8,0116,038,49
28,5139,3529,22
59,2452,361,5
24,1537,6624,79
MEANSDERR.
10,844,541,48
32,366,0618,74
57,684,798,31
28,877,6226,4
a1a2a3
18,7810,078,84
46,4529,6830,58
43,1553,0556,19
44,2926,4826,31
MEANSDERR.
12,565,4243,13
35,579,4326,52
50,86,8113,4
32,3610,3331,93