Team:Warsaw/Calendar-Stage1/17 August 2010
From 2010.igem.org
(Difference between revisions)
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<br><div class="note"> Testing the strenght of RBS [Ania O.,Cherry]</div> | <br><div class="note"> Testing the strenght of RBS [Ania O.,Cherry]</div> | ||
- | <br>Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer (all according the <a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">protocol</a> | + | <br>Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer (all according the <a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">protocol</a>) |
<br> | <br> | ||
<br><div class="note"> Data analysis with Cell Quest and Microsoft Excel[Cherry]</div> | <br><div class="note"> Data analysis with Cell Quest and Microsoft Excel[Cherry]</div> | ||
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<strong>Legend:</strong> | <strong>Legend:</strong> | ||
<hr /> | <hr /> | ||
- | <br />17-08-2010.001- TOP10 | + | <br />17-08-2010.001- TOP10 without construct |
<br />17-08-2010.015- <a href="http://partsregistry.org/Part:BBa_J61100">J61100</a> | <br />17-08-2010.015- <a href="http://partsregistry.org/Part:BBa_J61100">J61100</a> | ||
<br />17-08-2010.016- <a href="http://partsregistry.org/Part:BBa_J61101">J61101</a> | <br />17-08-2010.016- <a href="http://partsregistry.org/Part:BBa_J61101">J61101</a> |
Revision as of 19:35, 27 October 2010
17.08.2010 Tuesday
Previous day: Set up liquid cultures of E.coli TOP10 with measured comstructs [Ania O.]
We used a protocol described here
Testing the strenght of RBS [Ania O.,Cherry]
Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer (all according the protocol)
Data analysis with Cell Quest and Microsoft Excel[Cherry]
Legend:
17-08-2010.001- TOP10 without construct
17-08-2010.015- J61100
17-08-2010.016- J61101
17-08-2010.017- J61107
17-08-2010.018- J61117
17-08-2010.019- J61127
17-08-2010.020- B0030
17-08-2010.021- B0031
17-08-2010.022- B0032
17-08-2010.023- B0033
17-08-2010.024- B0034
For pdf version click here.
Cytometer data processed with Cell Quest:
Cell Quest data processed with Microsoft Excel:
geo mean- geometric mean of fluorescence of events in FL1
peak channel- nr of channel which recorded the biggest nr of events
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