Team:Warsaw/Calendar-Stage1/17 August 2010

From 2010.igem.org

(Difference between revisions)
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<br><div class="note"> Testing the strenght of RBS [Ania O.,Cherry]</div>
<br><div class="note"> Testing the strenght of RBS [Ania O.,Cherry]</div>
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<br>Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer (all according the <a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">protocol</a>
+
<br>Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer (all according the <a href="https://2010.igem.org/Team:Warsaw/Stage1/RBSMeas">protocol</a>)
<br>
<br>
<br><div class="note"> Data analysis with Cell Quest and Microsoft Excel[Cherry]</div>
<br><div class="note"> Data analysis with Cell Quest and Microsoft Excel[Cherry]</div>
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<strong>Legend:</strong>
<strong>Legend:</strong>
<hr />
<hr />
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<br />17-08-2010.001- TOP10
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<br />17-08-2010.001- TOP10 without construct
<br />17-08-2010.015- <a href="http://partsregistry.org/Part:BBa_J61100">J61100</a>
<br />17-08-2010.015- <a href="http://partsregistry.org/Part:BBa_J61100">J61100</a>
<br />17-08-2010.016- <a href="http://partsregistry.org/Part:BBa_J61101">J61101</a>
<br />17-08-2010.016- <a href="http://partsregistry.org/Part:BBa_J61101">J61101</a>

Revision as of 19:35, 27 October 2010

Example Tabs


17.08.2010 Tuesday

Previous day: Set up liquid cultures of E.coli TOP10 with measured comstructs [Ania O.]

We used a protocol described here

Testing the strenght of RBS [Ania O.,Cherry]

Preparation of probes by dilluting the bacteria cultures and examining them in flow cytometer (all according the protocol)

Data analysis with Cell Quest and Microsoft Excel[Cherry]

Legend:

17-08-2010.001- TOP10 without construct
17-08-2010.015- J61100
17-08-2010.016- J61101
17-08-2010.017- J61107
17-08-2010.018- J61117
17-08-2010.019- J61127
17-08-2010.020- B0030
17-08-2010.021- B0031
17-08-2010.022- B0032
17-08-2010.023- B0033
17-08-2010.024- B0034

For pdf version click here.


Cytometer data processed with Cell Quest:




Cell Quest data processed with Microsoft Excel:



geo mean- geometric mean of fluorescence of events in FL1
peak channel- nr of channel which recorded the biggest nr of events

partgeo meanpeak channel
allm1allm1
J611004,145,6416
J6110119,9834,77146
J6110720,5634,33145
J611175,9214,71111
J611277,3210,0619
B003099,78180,661254
B003143,566,73169
B003228,1546,28148
B003386,21152,421205
B003428,4841,65145
partgeo mean %B0033peak channel %B0033
allm1allm1
J611000,0480,03710,0293
J611010,23180,228110,2244
J611070,23850,225210,2195
J611170,06870,096510,0537
J611270,08490,06610,0439
B00301,15741,185311,239
B00310,50460,437810,3366
B00320,32650,303610,2341
B00331111
B00340,33040,273310,2195