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Team:Warsaw/Calendar-Stage1/10 September 2010
From 2010.igem.org
10.09.2010 Friday
Note: We're not satisfied with the results, because of some problems with the timetable of usage of the cytometer
Previous 2 days: Set up and culturing Hela cell culture [Kamil]
Use a protocol...
Previous day: Set up liquid cultures of different types of E.coli [Milena]
Use a protocol...
Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]
1. Incubation of Hela cells with different E.coli types
2. Preparation of probes with incubated cells (use of lysozyme) and examining them in flow cytometer
Data analysis with Cell Quest [Cherry]
legend:
E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP
Hela cells with bacteria:
ML.009- without bacteriaN
ML.010- TOP (50)
ML.003- G (25)
ML.004- I (25)
ML.008- I (50)
ML.011- G (50)
10-09-2010.001- J (50)
10-09-2010.002- A (50)
10-09-2010.003- J (25)
Data from the flow cytometer processed by Cell Quest:
