Team:Warsaw/Calendar-Stage1/10 September 2010

From 2010.igem.org

(Difference between revisions)
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<br> 10.09.2010 Friday
<br> 10.09.2010 Friday
<br><br>Note: We're not satisfied with the results, because of some problems with the timetable of usage of the cytometer  
<br><br>Note: We're not satisfied with the results, because of some problems with the timetable of usage of the cytometer  
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<br>
 
<br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div>
<br><br><div class="note"> Previous 2 days: Set up and culturing Hela cell culture [Kamil]</div>
<br> Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)
<br> Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (<a href="http://openwetware.org/wiki/The_BioBricks_Foundation:RFC#BBF_RFC_59:_Quantitative_measurement_of_mamallian_cell_invasion_by_bacteria_using_flow_cytometry">RFC 59 standard</a> point 5)

Revision as of 18:29, 27 October 2010

Example Tabs


10.09.2010 Friday

Note: We're not satisfied with the results, because of some problems with the timetable of usage of the cytometer

Previous 2 days: Set up and culturing Hela cell culture [Kamil]

Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (RFC 59 standard point 5)

Previous day: Set up liquid cultures of different types of E.coli [Ania P.]

Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (RFC 59 standard point 5)

Testing the invasivnes of E.coli in Hela cells [Kamil,Cherry]

1. Incubation of Hela cells with different E.coli types.
2. Preparation of probes with incubated cells and examining them in flow cytometer.
Conducted according to the protocol included in "Quantitative measurement of mamallian cell invasion by bacteria using flow cytometry" standard (RFC 59 standard points 6 and 7)

Data analysis with Cell Quest and Microsoft Excel [Cherry]


legend:

E.coli constructs:
G- GFP
I- Invasin+GFP
J- JPH+Listeriolysin O+Invasin+GFP
A- APH+Listeriolysin O+Invasin+GFP

Hela cells with bacteria:
ML.009- without bacteriaN
ML.010- TOP (50)
ML.003- G (25)
ML.004- I (25)
ML.008- I (50)
ML.011- G (50)
10-09-2010.001- J (50)
10-09-2010.002- A (50)
10-09-2010.003- J (25)



Data from the flow cytometer processed by Cell Quest: