Team:Utah State

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          <td bgcolor="#262626"><p align="center" style="font-size:20px; color:#FFFFFF;"> <a class="sideLinks" href="https://2010.igem.org/Team:Utah_State"> <em>CyanoBricks</em></a></p></td>
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            <td><p>The future of synthetic biology lies in expanding our ability to   engineer genes in new organisms. Our project develops a system to   engineer the genome of the photosynthetic cyanobacterium Synechocystis   sp. PCC6803, establishes expression standards for this species, and adds   a set of characterized Synechocystis promoters and ribosome binding   sites to the BioBrick toolbox. We developed a BioBrick vector that can   be used to assemble parts and devices in E. coli. Upon transformation   into Synechocystis, it integrates the device directly into the genome   through homologous recombination. We utilized genes that were activated   under a variety of conditions, from those responding to heat stress to   ones oscillating under a circadian rhythm. The promoters and ribosome   binding sites were converted into BioBrick-compatible parts, and   subsequently characterized. Our success will enable the use of existing   parts in new species, and will expand the range of devices that can be   built. </p></td>
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The future of synthetic biology lies in expanding our ability to engineer genes in new organisms. Our project develops a system to engineer the genome of the photosynthetic cyanobacterium Synechocystis sp. PCC6803, establishes expression standards for this species, and adds a set of characterized Synechocystis promoters and ribosome binding sites to the BioBrick toolbox. We developed a BioBrick vector that can be used to assemble parts and devices in E. coli. Upon transformation into Synechocystis, it integrates the device directly into the genome through homologous recombination. We utilized genes that were activated under a variety of conditions, from those responding to heat stress to ones oscillating under a circadian rhythm. The promoters and ribosome binding sites were converted into BioBrick-compatible parts, and subsequently characterized. Our success will enable the use of existing parts in new species, and will expand the range of devices that can be built.  
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Revision as of 04:52, 17 October 2010

Untitled Document

Welcome!

The future of synthetic biology lies in expanding our ability to engineer genes in new organisms. Our project develops a system to engineer the genome of the photosynthetic cyanobacterium Synechocystis sp. PCC6803, establishes expression standards for this species, and adds a set of characterized Synechocystis promoters and ribosome binding sites to the BioBrick toolbox. We developed a BioBrick vector that can be used to assemble parts and devices in E. coli. Upon transformation into Synechocystis, it integrates the device directly into the genome through homologous recombination. We utilized genes that were activated under a variety of conditions, from those responding to heat stress to ones oscillating under a circadian rhythm. The promoters and ribosome binding sites were converted into BioBrick-compatible parts, and subsequently characterized. Our success will enable the use of existing parts in new species, and will expand the range of devices that can be built.