Team:Utah State

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            <b><i>Welcome!</b></i></font>
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<font face="Century Gothic, Arial, San Serif" color =#ffffff><p align="left">The future of synthetic biology lies in expanding our ability to  engineer genes in new organisms. Our project develops a system to  engineer the genome of the photosynthetic cyanobacterium Synechocystis  sp. PCC6803, establishes expression standards for this species, and adds  a set of characterized Synechocystis promoters and ribosome binding  sites to the BioBrick toolbox. We developed a BioBrick vector that can  be used to assemble parts and devices in E. coli. Upon transformation  into Synechocystis, it integrates the device directly into the genome  through homologous recombination. We utilized genes that were activated  under a variety of conditions, from those responding to heat stress to  ones oscillating under a circadian rhythm. The promoters and ribosome  binding sites were converted into BioBrick-compatible parts, and  subsequently characterized. Our success will enable the use of existing  parts in new species, and will expand the range of devices that can be built. </p></font>
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''The aim of the Utah State University iGEM project is to develop flexible and adaptable systems for manufacturing cellular products using the standardized BioBrick system in a wide range of species. First, we altered the standard BioBrick vector pSB1A3 to allow for the insertion of genomic integration sequences from Synechocystis sp. PCC 6803 and enable the insertion of BioBrick constructs directly into the host genome. This integration vector is easily adapted for use in other species through the exchange of the species-specific integration sequences and the ability to alter the selection marker, if necessary. This vector will facilitate exploitation of advantageous characteristics of a wide range of organisms, including photosynthetic carbon assimilation. The BioBrick toolbox was expanded to allow the utilization of Synechocystis as a host species, with the identification and characterization of ribosome binding sites, constitutive promoters, and promoters responsive to light, dark, nitrogen stress, heat stress, and circadian rhythms. Secretion systems for Synechocystis were adapted to the BioBrick standard, and the cross-species function of E. coli secretion tags was investigated. Project success will facilitate the expansion of BioBrick-coded products into multiple organisms and the characterization of further useful genetic elements in those species.''
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[[Image:charles.jpg|center|frame|Dr. Charles Miller|50px]]
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[[Image:cody.jpg|center|frame|Cody Tramp|50px]]
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[[Image:brad.jpg|center|frame|Brad Henrie|50px]]
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[[Image:shujie.jpg|center|frame|Shujie Shen|50px]]
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[[Image:Abiezer.jpg|center|frame|Abiezer Tejeda|50px]]
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[[Image:Alex.jpg|center|frame|Alex Hatch|50px]]
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[[Image:Eduardo.jpg|center|frame|Eduardo Monzon|50px]]
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<!--- The Mission, Experiments --->
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!align="center"|[[Team:Utah_State|Home]]
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!align="center"|[[Team:Utah_State/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Utah_State Official Team Profile]
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!align="center"|[[Team:Utah_State/Project|Project]]
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!align="center"|[[Team:Utah_State/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Utah_State/Modeling|Modeling]]
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!align="center"|[[Team:Utah_State/Notebook|Notebook]]
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!align="center"|[[Team:Utah_State/Safety|Safety]]
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Latest revision as of 00:07, 28 October 2010

USU_IGEM

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Welcome!

The future of synthetic biology lies in expanding our ability to engineer genes in new organisms. Our project develops a system to engineer the genome of the photosynthetic cyanobacterium Synechocystis sp. PCC6803, establishes expression standards for this species, and adds a set of characterized Synechocystis promoters and ribosome binding sites to the BioBrick toolbox. We developed a BioBrick vector that can be used to assemble parts and devices in E. coli. Upon transformation into Synechocystis, it integrates the device directly into the genome through homologous recombination. We utilized genes that were activated under a variety of conditions, from those responding to heat stress to ones oscillating under a circadian rhythm. The promoters and ribosome binding sites were converted into BioBrick-compatible parts, and subsequently characterized. Our success will enable the use of existing parts in new species, and will expand the range of devices that can be built.

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