Team:UT-Tokyo/Sudoku protocols

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Sudoku

Protocols and data

This is an abstract page. If you can see detail data, please click each protocols. This is an abstract page. If you can see detail data, please click each protocols.

Pre-experiment

Pre-experiment

Make construct

0,i,ii,iii

Check test

Check test


Number Name Link to BioBrick Plate coordinate Vector Code length
1 T7 promoter

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]

plate1-6N pSB1AK8 46bp
2 rbs

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030]

plate1-1H pSB1A2 15bp
3 cre recombinase

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61047 BBa_J61047]

plate1-5D pSB1A2 1037bp
4 double terminator

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014]

plate2-24C pSB1AK3 95bp
5 lox66 recombinase site

[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718017 BBa_I718017]

plate1-17J pSB1A2 34bp
6 Kan resistance (rev)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31002 BBa_J31002]

plate1-2K pSB1A2 816bp
7 rbs (rev)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014]

plate1-1J pSB1A2 15bp
8 lox71 recombinase site

order-made

--- --- 34bp
9 single terminator

[http://partsregistry.org/wiki/index.php?title=Part:BBa_B1006 BBa_B1006]

plate1-4H pSB1AK3 39bp
10 constant express promoter

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119]

plate1-18A pSB1A2 35bp
11 Tet resistance (rev)

[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006 BBa_J31006]

plate1-1N pSB1A2 1191bp


Main construct

Research of SP6 RNApol cDNA and F plasmid

Research of SP6 RNApol cDNA and F plasmid

Convert lox71(rev) to lox66(rev)

Convert lox71(rev) to lox66(rev)

Make Hin(rev)

Make Hin(rev)

Revise MS2 gene

Revise MS2 gene

Make location(rev)-rbs(rev)-location(rev)

Make location(rev)-rbs(rev)-location(rev)


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