Team:UT-Tokyo/Sudoku experiments

From 2010.igem.org

(Difference between revisions)
(flpe)
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== Hin ==
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===Parts making (reverse)===
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[[Image:Sudoku_Hin_making.png|200px|thumb|How to make Hin parts]]
 +
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We get Hin parts from HQ (BBa_J31000).
 +
 +
We use this part as the form of reverse, so we made this part reverse by PCR.
 +
 +
To check PCR is done correctly, we did VspI digestion.
 +
 +
 +
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===Expression check===
 +
[[Image:Sudoku_Assay_Hin.png|200px|thumb|Hin expression check assay]]
 +
 +
To check whether flpe works correctly, we made assay shown in Fig, similar to the assay of flpe check.
 +
 +
The top construct is a nagative control.
 +
GFP can't be expressed because of the double terminators.
 +
 +
The bottom construct express GFP when Hin works correctly.
 +
When Hin is expressed correctly, Hin recognaize the hix site and double terminator which terminates the expression of gfp is removed, so GFP may be expressed.
== flpe ==
== flpe ==
Line 127: Line 151:
When flpe is expressed correctly, flpe recognaize the frt site and double terminator which terminates the expression of gfp is removed, so GFP may be expressed.
When flpe is expressed correctly, flpe recognaize the frt site and double terminator which terminates the expression of gfp is removed, so GFP may be expressed.
-
== Hin ==
 
-
===Parts making (reverse)===
 
-
[[Image:Sudoku_Hin_making.png|200px|thumb|How to make Hin parts]]
 
-
 
-
We get Hin parts from HQ (BBa_J31000).
 
-
 
-
We use this part as the form of reverse, so we made this part reverse by PCR.
 
-
 
-
To check PCR is done correctly, we did VspI digestion.
 
-
 
-
 
-
 
-
===Expression check===
 
-
[[Image:Sudoku_Assay_Hin.png|200px|thumb|Hin expression check assay]]
 
-
 
-
To check whether flpe works correctly, we made assay shown in Fig, similar to the assay of flpe check.
 
-
 
-
The top construct is a nagative control.
 
-
GFP can't be expressed because of the double terminators.
 
-
 
-
The bottom construct express GFP when Hin works correctly.
 
-
When Hin is expressed correctly, Hin recognaize the hix site and double terminator which terminates the expression of gfp is removed, so GFP may be expressed.
 
= Parts Modeling =
= Parts Modeling =

Revision as of 17:09, 27 October 2010

UT-Tokyo

Sudoku

Introduction System Modeling Lab note Experiment Result Reference


Assay

1. #Terminator leak

2. #Phage MS2

3. #Location sequence

Parts Making

1. #Terminator leak

2. #Phage MS2

3. #Location Sequence

4. #Hin

5. #flpe

Parts Modeling

Detail Note Book

Assay

Terminator leak

Terminator leak switch assay

Phage MS2

Parts making

How to make MS2 parts

We get MS2 gene RT-PCR product. This original product include a lot of restricted enzyme site: two EcoRI site, two XbaI site and one VspI site.

To run our project, we don’t have to remove EcoRI site in the region. So we modified XbaI site by PCR as the following method:

1. We divided RT-PCR product into two parts by XP enzyme digestion:

E-X-E-E-V-X -> “Goten”

X-S-P -> “Tranks”

(named from the famous animation, "DRAGON BALL")

2. Insert is ligated with the vector, chloramphenicol-tolerance:

Goten -> EX vector

Tranks -> XP vector

3. Tranks -> PCR adding V-X region

4. Each part -> VP enzyme digestion, ligation each other


Expression check

Virus expression check assay

We use MS2 phage to transmit information of location and number. The expression of the phage should start after 4C3 leak switch turns on. MS2 phage transport RNA which has loding sequence, so we knock out self assembly and made our E.coli translate loading seaquence at another point. To check whether transportion go correctly, we made assay.

i. Phage expression assay (red region)

Check whether RT-PCR product can be translated into MS2 phage.

ii. Packaging assay (blue & green region)

MS2 phage package RNA which has loading sequence. Using this character, we check whether RNA which has loading sequence and other coding region (gfp, cre) can be packaged correctly.

iii. Infection assay (yellow & green region)

We make the assay, which express gfp only when cre protein is expressed correctly. By using this part, we check whether E.coli can be infected with the MS2 phage made in assay ii as the fluorescence of gfp.

Location sequence

The object of the assay is to test whether antisense RNA used in our construct works or not. In order to block the unnecessary information transformed by the virus from the other grid, we use antisense RNA to block ribosome to bind the region around the ribosome binding site (rbs) and prevent the expression of protein. In our construct, information is carried by virus and antisense RNA is transcribed constantly inside the cell. Once the unnecessary information was transformed, the antisense will come and shut out all the RNA chain excluded by virus. In this assay, we used pBAD as the promoter to start translating grid information which will be transformed by virus in our construct. The strength of the promoter depends on the concentration of arabinose. On the other hand, we used c-pro as the promoter to start translating antisense RNA. This c-pro is the strongest constitutive promoter submitted in igem parts. In assay I, we examined the relative strength of pBAD (with eight different concentration of arabinose) and c-pro. By using the proper concentration of arabinose which was determined by assay I, in assay II, we inspect observe whether our antisense RNA works or not. In these two assay, gfp was used as a reporter protein.



Hin

Parts making (reverse)

How to make Hin parts

We get Hin parts from HQ (BBa_J31000).

We use this part as the form of reverse, so we made this part reverse by PCR.

To check PCR is done correctly, we did VspI digestion.


Expression check

Hin expression check assay

To check whether flpe works correctly, we made assay shown in Fig, similar to the assay of flpe check.

The top construct is a nagative control. GFP can't be expressed because of the double terminators.

The bottom construct express GFP when Hin works correctly. When Hin is expressed correctly, Hin recognaize the hix site and double terminator which terminates the expression of gfp is removed, so GFP may be expressed.

flpe

How to make flpe parts

The original flpe include two restriction enzyme sites(EcoRI, SpeI).

We modified this flpe by PCR:

1st PCR : cloning

-> ligation with vector

-> 2nd PCR : modified SpeI site

-> 3rd PCR : modified EcoRI site

We use this part as the form of reverse, so we made this part reverse by PCR.

Expression check

flpe expression check assay

To check whether flpe works correctly, we made assay shown in Fig.

The top construct is a nagative control. GFP can't be expressed because of the double terminators.

The bottom construct express GFP when flpe works correctly. When flpe is expressed correctly, flpe recognaize the frt site and double terminator which terminates the expression of gfp is removed, so GFP may be expressed.


Parts Modeling

Detail Note Book

Parts list

Number Name Link to BioBrick Plate coordinate Vector Code length
1 T7 promoter

BBa_I712074

plate1-6N pSB1AK8 46bp
2 rbs

BBa_B0030

plate1-1H pSB1A2 15bp
3 cre recombinase

BBa_J61047

plate1-5D pSB1A2 1037bp
4 double terminator

BBa_B0014

plate2-24C pSB1AK3 95bp
5 lox66 recombinase site

BBa_I718017

plate1-17J pSB1A2 34bp
6 Kan resistance (rev)

BBa_J31002

plate1-2K pSB1A2 816bp
7 rbs (rev)

BBa_B0014

plate1-1J pSB1A2 15bp
8 lox71 recombinase site

order-made

--- --- 34bp
9 single terminator

BBa_B1006

plate1-4H pSB1AK3 39bp
10 constant express promoter

BBa_J23119

plate1-18A pSB1A2 35bp
11 Tet resistance (rev)

BBa_J31006

plate1-1N pSB1A2 1191bp
12 hixC

BBa_J44000

plate1-1B pSB1A2 26bp
13 ---

---

--- --- ---
14 lox66

BBa_I718016

plate1-17H pSB1A2 34bp
15 rbs-mRFP1-terminator

BBa_I13507

plate1-22O pSB1A2 861bp
16 location sequence(old)

order

--- --- ---
17 pSP6(rev)

order

--- --- ---
18 lox2272

order

--- --- ---
19 loading sequence(rev)

order

--- --- ---
20 frt

BBa_J61020

From HQ pSB1A2 34bp
21 hin(true)

BBa_J31000

From HQ  ??? 573bp
22 hin(rev)

---

---  ??? 573bp
23 flpe

---

--- --- about 1.2kbp
24 gfp unit

---

--- --- about 1.2kbp
25 flpe(rev)

---

--- --- about 1.2kbp