Protocols

Notice

-You should mix reagents well

-Put in enzymes after you mixed all other reagents

-Centrifuge the tube and collect substances on the bottom before opening a reagent

Transformation

Colony Pick Up

Miniprep

Restriction Enzyme digestion（Xba1/Pst1）—Once for all

Restriction Enzyme digestion —One by one

PCR(Pfu Ultra)

PCR（Ex-Taq）

PCR（KODplus）

Colony PCR（Ex-Taq）

Electrophoresis

Gel DNA Recovery

LB　broth

Agarose Gel

Seaquencing

Producing Competent Cell