Team:UT-Tokyo/Protocols

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UT-Tokyo

Protocols

Notice

-You should mix reagents well

-Put in enzymes after you mixed all other reagents

-Centrifuge the tube and collect substances on the bottom before opening a reagent


Transformation Colony Pick Up

Miniprep

Restriction Enzyme Transaction(Xba1/Pst1)—Once for all

Restriction Enzyme Transaction —One by one

PCR(Pfu Ultra)

PCR(Ex-Taq)

PCR(KODplus)

Colony PCR(Ex-Taq)

Electrophoresis

Gel DNA Recovery

LB broth

Agarose Gel

Seaquencing

Producing Competent Cell