Team:UT-Tokyo/Protocols

Protocols

Notice

-You should mix reagents well -Put in enzymes after you mixed all other reagents -Centrifuge the tube and collect substances on the bottom before opening a reagent

Transformation Colony Pick Up Miniprep Restriction Enzyme Transaction（Xba1/Pst1）—Once for all Restriction Enzyme Transaction —One by one PCR（Pfu　Ultra） PCR（Ex-Taq） PCR（KODplus） Colony PCR（Ex-Taq） Electrophoresis Gel DNA Recovery LB　broth Agarose Gel Seaquencing Producing Competent Cell