Team:UT-Tokyo/Protocols

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UT-Tokyo

! Notice ! -You should mix reagents well -Put in enzymes after you mixed all other reagents -Centrifuge the tube and collect substances on the bottom before opening a reagent

Transformation Colony Pick Up Miniprep Restriction Enzyme Transaction(Xba1/Pst1)—Once for all Restriction Enzyme Transaction —One by one PCR(Pfu Ultra) PCR(Ex-Taq) PCR(KODplus) Colony PCR(Ex-Taq) Electrophoresis Gel DNA Recovery LB broth Agarose Gel Seaquencing Producing Competent Cell