Protocols

Notice:

-You should mix reagents well -Put in enzymes after you mixed all other reagents -Centrifuge the tube and collect substances on the bottom before opening a reagent

• Transformation

• Colony Pick Up

• Miniprep

• Restriction Enzyme digestion（Xba1/Pst1）—Once for all

• Restriction Enzyme digestion —One by one

• PCR(Pfu Ultra)

• PCR（Ex-Taq）

• PCR（KODplus）

• Colony PCR（Ex-Taq）

• Electrophoresis

• Gel DNA Recovery

• LB　broth

• Agarose Gel

• Seaquencing

• Producing Competent Cell